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Using a Genetically Encoded FRET-Based Reporter to Visualize Calcineurin Phosphatase Activity in Living Cells

  • Sohum Mehta
  • Jin Zhang
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1071)

Abstract

Calcineurin is an evolutionarily conserved, ubiquitously expressed protein phosphatase that serves as a major effector of Ca2+ signals, regulating diverse biological processes such as gene expression, tissue differentiation, immune responses, and neural plasticity. The following method describes how to monitor real-time calcineurin activity in cultured mammalian cells using a fluorescence resonance energy transfer (FRET)-based activity reporter.

Key words

Calcineurin Phosphatase activity Biosensor Calmodulin NFAT FRET Live-cell imaging 

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Copyright information

© Springer Science+Business Media, LLC 2014

Authors and Affiliations

  • Sohum Mehta
    • 1
  • Jin Zhang
    • 1
  1. 1.Department of Pharmacology and Molecular SciencesThe Johns Hopkins University School of MedicineBaltimoreUSA

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