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Tandem Mass Spectrometry in Hormone Measurement

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Hormone Assays in Biological Fluids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1065))

Abstract

Mass spectrometry methods have the potential to measure different hormones during the same analysis and have improved specificity and a wide analytical range compared with many immunoassay methods. Increasingly in clinical laboratories liquid chromatography-tandem mass spectrometry (LC-MS/MS) assays are replacing immunoassays for the routine measurement of testosterone, 17-hydroxyprogesterone, and other steroid hormones. Reference LC-MS/MS methods for steroid, thyroid, and peptide hormones are being used for assessment of the performance and calibration of commercial immunoassays. In this chapter, the general principles of tandem mass spectrometry and examples of hormone assays are described.

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  1. Department of Specialist Laboratory Medicine, St. James’s University Hospital, Leeds, UK

    Helen P. Field

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  1. Consultant Clinical Scientist The Old Dairy, Exmouth, United Kingdom

    Michael J. Wheeler

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Field, H.P. (2013). Tandem Mass Spectrometry in Hormone Measurement. In: Wheeler, M. (eds) Hormone Assays in Biological Fluids. Methods in Molecular Biology, vol 1065. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-616-0_4

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