Introduction to Gas Chromatography-Mass Spectrometry
Gas chromatography-mass spectrometry (GC-MS) is a technique of pivotal importance for the analysis of hormones in biological fluids. In consequence, it has gained relevance in clinical and endocrinological laboratories, providing reference analytical methods. This chapter offers a general description of its principles, and a real example for GC-MS profiling of plasma steroids.
Key wordsGas chromatography Mass spectrometry Derivatization Isotopic labeling Steroids Plasma
The excellent technical assistance of Mrs. Birgit Wardega is gratefully appreciated. Stefan A. Wudy thankfully acknowledges funding from the “Deutsche Forschungsgemeinschaft (DFG)” during the last 20 years, having enabled constant progression in analytical method development.
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