Abstract
Gas chromatography-mass spectrometry (GC-MS) is a technique of pivotal importance for the analysis of hormones in biological fluids. In consequence, it has gained relevance in clinical and endocrinological laboratories, providing reference analytical methods. This chapter offers a general description of its principles, and a real example for GC-MS profiling of plasma steroids.
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References
Makin HLJ, Gower DB (eds) (2010) Steroid analysis, 2nd edn. Springer, London
Grob RL, Barry EF (eds) (2004) Modern practice of gas chromatography, 4th edn. Wiley, Hoboken, NJ
Shackleton CHL (1985) Mass spectrometry: application to steroid and peptide research. Endocr Rev 6:441–486
Wudy SA, Hartmann MF (2004) Gas chromatography-mass spectrometry profiling of steroids in times of molecular biology. Horm Metab Res 36:415–422
Wudy SA (1990) Synthetic procedures for the preparation of deuterium-labeled analogs of naturally occurring steroids. Steroids 55:463–471
Wudy SA, Hartmann M, Homoki J (2002) Determination of 11-deoxycortisol (Reichstein’s compound S) in human plasma by clinical isotope dilution mass spectrometry using benchtop gas chromatography-mass selective detection. Steroids 67:851–857
Wudy SA, Wachter UA, Homoki J, Teller WM, Shackleton CH (1992) Androgen metabolism assessment by routine gas chromatography/mass spectrometry profiling of plasma steroids. Part 1, unconjugated steroids. Steroids 57:319–324
Acknowledgments
The excellent technical assistance of Mrs. Birgit Wardega is gratefully appreciated. Stefan A. Wudy thankfully acknowledges funding from the “Deutsche Forschungsgemeinschaft (DFG)” during the last 20 years, having enabled constant progression in analytical method development.
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Sánchez-Guijo, A., Hartmann, M.F., Wudy, S.A. (2013). Introduction to Gas Chromatography-Mass Spectrometry. In: Wheeler, M. (eds) Hormone Assays in Biological Fluids. Methods in Molecular Biology, vol 1065. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-616-0_3
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DOI: https://doi.org/10.1007/978-1-62703-616-0_3
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-615-3
Online ISBN: 978-1-62703-616-0
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