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Introduction to Gas Chromatography-Mass Spectrometry

  • Alberto Sánchez-Guijo
  • Michaela F. Hartmann
  • Stefan A. Wudy
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1065)

Abstract

Gas chromatography-mass spectrometry (GC-MS) is a technique of pivotal importance for the analysis of hormones in biological fluids. In consequence, it has gained relevance in clinical and endocrinological laboratories, providing reference analytical methods. This chapter offers a general description of its principles, and a real example for GC-MS profiling of plasma steroids.

Key words

Gas chromatography Mass spectrometry Derivatization Isotopic labeling Steroids Plasma 

Notes

Acknowledgments

The excellent technical assistance of Mrs. Birgit Wardega is gratefully appreciated. Stefan A. Wudy thankfully acknowledges funding from the “Deutsche Forschungsgemeinschaft (DFG)” during the last 20 years, having enabled constant progression in analytical method development.

References

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Alberto Sánchez-Guijo
    • 1
  • Michaela F. Hartmann
    • 1
  • Stefan A. Wudy
    • 2
  1. 1.Laboratory for Translational Hormone Analytics in Paediatric Endocrinology, Steroid Research & Mass Spectrometry Unit, Division of Paediatric Endocrinology & DiabetologyJustus Liebig UniversityGiessenGermany
  2. 2.Division of Paediatric Endocrinology & Diabetology, Laboratory for Translational Hormone Analytics in Paediatric Endocrinology, Steroid Research & Mass Spectrometry UnitJustus Liebig UniversityGiessenGermany

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