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Measurement of Plasma Renin Activity

  • Sophie C. Barnes
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1065)

Abstract

This chapter describes the measurement of plasma renin activity by calculation of the quantity of angiotensin I that is released in plasma by the action of endogenous enzyme renin on angiotensinogen, the endogenous substrate. Details are given for the generation of angiotensin I from plasma using controlled pH and temperature conditions. The generated angiotensin I is then quantified by a radioimmunoassay using anti-angiotensin I antibody and iodinated angiotensin I as label. Separation of the antibody-bound fraction from the unbound is achieved using dextran-coated charcoal. Additional notes describe critical steps in the assay. The correct procedures for sample handling to overcome the potential problems of cryoactivation of prorenin to renin are described.

Key words

Renin Prorenin Cryoactivation Radioimmunoassay Dextran-coated charcoal Angio-tensinogen Angiotensin I Angiotensin-converting enzyme 

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Sophie C. Barnes
    • 1
  1. 1.Imperial College Healthcare NHS TrustCharing Cross HospitalLondonUK

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