Measurement of Aldosterone in Blood

  • Sophie C. Barnes
Part of the Methods in Molecular Biology book series (MIMB, volume 1065)


This chapter describes the measurement of aldosterone concentration in plasma or serum. Aldosterone specificity is improved by first extracting the aldosterone into dichloromethane. A radioimmunoassay is then performed on the dried and reconstituted extract using an anti-aldosterone antibody and iodinated aldosterone. The antibody-bound fraction is separated from the unbound fraction using activated charcoal. The notes section provides extra information for steps in the assay that can be problematic.

Key words

Aldosterone Mineralocorticoid Solvent extraction Radioimmunoassay Dextran-coated charcoal 



With thanks to Mike Scanlon for his expertise and careful review of this and the renin activity chapter.


  1. 1.
    Hiramatsu K, Yamada T, Yukimura Y (1981) A screening test to identify aldosterone producing adenoma by measuring plasma renin activity: results in hypertensive patients. Arch Intern Med 141:1589–1593PubMedCrossRefGoogle Scholar
  2. 2.
    Funder JW, Carey RM, Fardella C et al (2008) Case detection, diagnosis, and treatment of patients with primary aldosteronism: an endocrine society clinical practice guideline. J Clin Endocrinol Metab 93:3266–3281PubMedCrossRefGoogle Scholar
  3. 3.
    Stockl D, Reinauer H, Thienpoint LM et al (1991) Determination of aldosterone in human serum by isotope dilution gas chromatography/mass spectrometry using a new heptaflurobutyryl derivative. Biol Mass Spectrom 20:657–664PubMedCrossRefGoogle Scholar
  4. 4.
    Prome D, Viger A, Marquet A (1988) Use of tandem mass spectrometry (MS-MS) for aldosterone assay at the nanogram level in complex biological mixtures. Anal Biochem 172:264–269PubMedCrossRefGoogle Scholar
  5. 5.
    Miller MA, Sagnella GA, Macgregor GA (1997) Extracted method and non-extracted kit method compared for measuring plasma aldosterone. Clin Chem 43:1995–1997PubMedGoogle Scholar
  6. 6.
    Hartman D, Doulton TW, Chesters CA et al (2006) Plasma aldosterone: comparison of a new automated assay with a standard extraction method. Clin Chem 52:2118–2119PubMedCrossRefGoogle Scholar
  7. 7.
    Selby C (1999) Interference in immunoassays. Ann Clin Biochem 36:704–721PubMedGoogle Scholar
  8. 8.
    Kricka LJ (2000) Interference in immunoassays-still a threat. Clin Chem 46:1037–1038PubMedGoogle Scholar
  9. 9.
    Jones JC, Carter GD, MacGregor GA (1981) Interference by polar metabolites in a direct radioimmunoassay for plasma aldosterone. Ann Clin Biochem 18:54–59PubMedGoogle Scholar
  10. 10.
    Few JD, Chaudry S, James VHT (1984) The direct determination of aldosterone in human saliva. J Steroid Biochem 21:87–92PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Sophie C. Barnes
    • 1
  1. 1.Imperial College Healthcare NHS TrustCharing Cross HospitalLondonUK

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