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Analysis of In Vivo Occupancy of Aebp1, a Transcription Factor, Using High Resolution Tiling Array

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Tiling Arrays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1067))

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Abstract

Aebp1 is a transcription factor that negatively regulates adipogenesis and also is upregulated in primary GBM as against secondary GBM. Here we describe a systematic approach to map the in vivo occupancy sites of Aebp1 by using a combination of chromatin immune-precipitation technique and hybridization to Agilent high resolution human promoter tiling array (2×224) containing 474393 probes. We also describe the use of various bioinformatics tools for analysis of the hybridization data and identification of Aebp1 occupancy sites in U87MG glioma cell line.

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Acknowledgments

This work was supported by grants from NMITLI program of Council of Scientific and Industrial Research (CSIR) and Department of Biotechnology (DBT), New Delhi.

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Sinha, S., Donakonda, S., Rao, S.M. (2013). Analysis of In Vivo Occupancy of Aebp1, a Transcription Factor, Using High Resolution Tiling Array. In: Lee, TL., Shui Luk, A. (eds) Tiling Arrays. Methods in Molecular Biology, vol 1067. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-607-8_11

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  • DOI: https://doi.org/10.1007/978-1-62703-607-8_11

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-606-1

  • Online ISBN: 978-1-62703-607-8

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