Cell Membrane Vesicles as a Tool for the Study of Direct Epithelial–Stromal Interaction: Lessons from CD147

  • Eric Gabison
  • Farah Khayati
  • Samia Mourah
  • Suzanne Menashi
Part of the Methods in Molecular Biology book series (MIMB, volume 1066)


Communication between the epithelial and stromal tissue layers, separated by basement membrane, is known to provide the information necessary for development, differentiation, and homeostasis. These interactions are altered in benign or malignant diseases, in particular when the basement membrane barrier is disrupted allowing a greater proximity between the two cell layers that triggers tissue remodeling. Epithelial–stromal interactions (ESI) have been examined in vitro by various approaches that can be broadly divided into interactions arising from secreted diffusible factors and interactions through direct cell–cell contact. Here we describe a method for the study of direct ESI through CD147, an adhesion molecule present on the epithelial cell surface and which is known to interact with stromal cells, such as fibroblasts and endothelial cells, and signal them to increase production of matrix metalloproteinases. This method can be extended to other adhesion molecules involved in ESI.

Key words

Tumor–stroma interactions CD147 Membrane vesicles Stromal cells Epithelial cells 


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Copyright information

© Springer Science+Business Media, New York 2013

Authors and Affiliations

  • Eric Gabison
    • 1
  • Farah Khayati
    • 2
  • Samia Mourah
    • 2
  • Suzanne Menashi
    • 3
  1. 1.Univ Paris Diderot and Fondation A. de RothschildParisFrance
  2. 2.Laboratoire de Pharmacologie and INSERM U940Hôpital Saint- LouisParisFrance
  3. 3.CNRS EAC 7149, Faculté des SciencesUniversité Paris-Est CréteilCréteilFrance

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