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Methods to Assess Tissue Permeability

  • Juan C. Ibla
  • Joseph Khoury
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1066)

Abstract

An essential requirement for adequate organ performance is the formation of permeability barriers that separate and maintain compartments of distinctive structure and function. The endothelial cell lining of the vasculature defines a semipermeable barrier between the blood and interstitial spaces of all organs. Disruption of the endothelial cell barrier can result in increased permeability and vascular leak. These effects are associated with multiple systemic disease processes and can accompany acute tissue responses to injury. The mechanisms that control barrier function are complex and their full understanding requires a multidisciplinary approach. The use of in vivo permeability data often complements molecular findings and adds power to the studies. The interaction of multiple cell types and tissues present only in mammalian models allows for testing of hypothesis and establishing the physiological significance of the results. In this chapter we describe simple methods that can be used systematically to measure the permeability profile of several organs.

Key words

Permeability assay Vasculature Edema Inflammation Evans blue dye Fluorescence bioparticles Water content Wet-to-dry ratio 

References

  1. 1.
    Beck KF, Eberhardt W, Frank S et al (1999) Inducible NO synthase: role in cellular signalling. J Exp Biol 202:645–653PubMedGoogle Scholar
  2. 2.
    Bertuglia S, Giusti A (2005) Role of nitric oxide in capillary perfusion and oxygen delivery regulation during systemic hypoxia. Am J Physiol Heart Circ Physiol 288: H525–H531PubMedCrossRefGoogle Scholar
  3. 3.
    Hofmann T, Stutts MJ, Ziersch A et al (1998) Effects of topically delivered benzamil and amiloride on nasal potential difference in cystic fibrosis. Am J Respir Crit Care Med 157:1844–1849PubMedCrossRefGoogle Scholar
  4. 4.
    Karhausen J, Ibla JC, Colgan SP (2003) Implications of hypoxia on mucosal barrier function. Cell Mol Biol (Noisy-le-grand) 49:77–87Google Scholar
  5. 5.
    Bleeker-Rovers CP, Boerman OC, Rennen HJ et al (2004) Radiolabeled compounds in diagnosis of infectious and inflammatory disease. Curr Pharmal Design 10:2935–2950CrossRefGoogle Scholar
  6. 6.
    Miles AA, Miles EM (1952) Vascular reactions to histamine, histamine-liberator and leukotaxine in the skin of guinea-pigs. J Physiol 118:228–257PubMedGoogle Scholar
  7. 7.
    Cui B, Sun JH, Xiang FF et al (2012) Aquaporin 4 knockdown exacerbates streptozotocin-induced diabetic retinopathy through aggravating inflammatory response. Exp Eye Res 98:37–43PubMedCrossRefGoogle Scholar
  8. 8.
    Liu K, Sun T, Wang P et al (2013) Effects of erythropoietin on blood–brain barrier tight junctions in ischemia-reperfusion rats. J Mol Neurosci 49:369–379PubMedCrossRefGoogle Scholar
  9. 9.
    Chintagari NR, Liu L (2012) GABA receptor ameliorates ventilator-induced lung injury in rats by improving alveolar fluid clearance. Crit Care 16:R55PubMedCrossRefGoogle Scholar
  10. 10.
    Rudolph AM, Heymann MA (1967) The circulation of the fetus in utero. Methods for studying distribution of blood flow, cardiac output and organ blood flow. Circ Res 21: 163–184PubMedCrossRefGoogle Scholar
  11. 11.
    Rosenberger P, Khoury J, Kong T et al (2007) Identification of vasodilator-stimulated phosphoprotein (VASP) as an HIF-regulated tissue permeability factor during hypoxia. FASEB J 21:2613–2621PubMedCrossRefGoogle Scholar
  12. 12.
    Lu M, Munford RS (2011) The transport and inactivation kinetics of bacterial lipopolysaccharide influence its immunological potency in vivo. J Immunol 187:3314–3320PubMedCrossRefGoogle Scholar
  13. 13.
    Wei SC, Hsu PH, Lee YF et al (2012) Selective detection of iodide and cyanide anions using gold-nanoparticle-based fluorescent probes. ACS Appl Mater Interfaces 4:2652–2658PubMedCrossRefGoogle Scholar
  14. 14.
    Synnestvedt K, Furuta GT, Comerford KM et al (2002) Ecto-5′-nucleotidase (CD73) regulation by hypoxia-inducible factor-1 mediates permeability changes in intestinal epithelia. J Clin Invest 110:993–1002PubMedGoogle Scholar
  15. 15.
    Samel S, Keese M, Kleczka M et al (2002) Microscopy of bacterial translocation during small bowel obstruction and ischemia in vivo—a new animal model. BMC Surg 2:6PubMedCrossRefGoogle Scholar
  16. 16.
    Sorrells DL, Friend C, Koltuksuz U et al (1996) Inhibition of nitric oxide with aminoguanidine reduces bacterial translocation after endotoxin challenge in vivo. Arch Surg 131:1155–1163PubMedCrossRefGoogle Scholar
  17. 17.
    Thompson LF, Eltzschig HK, Ibla JC et al (2004) Crucial role for ecto-5′-nucleotidase (CD73) in vascular leakage during hypoxia. J Exp Med 200:1395–1405PubMedCrossRefGoogle Scholar
  18. 18.
    Toung TJ, Chang Y, Lin J et al (2005) Increases in lung and brain water following experimental stroke: effect of mannitol and hypertonic saline. Crit Care Med 33:203–208, discussion 259–260PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, New York 2013

Authors and Affiliations

  • Juan C. Ibla
    • 1
  • Joseph Khoury
    • 2
  1. 1.Department of Pediatrics, Genetics Medicine and Integrative Systems BiologyGeorge Washington UniversityWashington, DCUSA
  2. 2.Department of Cellular and Molecular BiologyExogenesis CorporationBillericaUSA

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