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Generation and Analysis of Biosensors to Measure Mechanical Forces Within Cells

  • Katharina Austen
  • Carleen Kluger
  • Andrea Freikamp
  • Anna Chrostek-Grashoff
  • Carsten Grashoff
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1066)

Abstract

The inability to measure mechanical forces within cells has been limiting our understanding of how mechanical information is processed on the molecular level. In this chapter, we describe a method that allows the analysis of force propagation across distinct proteins within living cells using Förster resonance energy transfer (FRET)-based biosensors.

Key words

Mechanobiology Mechanotransduction Force measurement Biosensor Tension sensor Förster resonance energy transfer (FRET) Fluorescence-lifetime imaging microscopy (FLIM) 

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Copyright information

© Springer Science+Business Media, New York 2013

Authors and Affiliations

  • Katharina Austen
    • 1
  • Carleen Kluger
    • 1
  • Andrea Freikamp
    • 1
  • Anna Chrostek-Grashoff
    • 1
  • Carsten Grashoff
    • 1
  1. 1.Group of Molecular MechanotransductionMax-Planck-Institute of BiochemistryMartinsriedGermany

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