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Measuring Cell–Cell Tugging Forces Using Bowtie-Patterned mPADs (Microarray Post Detectors)

  • Daniel M. Cohen
  • Mike T. Yang
  • Christopher S. Chen
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1066)

Abstract

Cells generate traction forces upon adhesion to the extracellular matrix as well as to neighboring cells. These forces are important for the growth and maintenance of adhesion structures such as focal adhesions and adherens junctions, and may play roles in tissue development. Here, we describe a method for measuring the tugging force transmitted across the cell–cell junction between two paired cells.

Key words

Cell–cell tugging force Cell–cell junctions Micropatterning mPADs Traction force 

Notes

Acknowledgements

This work was supported by NIH grants HL73305 and through the RESBIO program (Integrated Technology Resource for Polymeric Biomaterials, NIH grant EB001046).

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Copyright information

© Springer Science+Business Media, New York 2013

Authors and Affiliations

  • Daniel M. Cohen
    • 1
  • Mike T. Yang
    • 1
  • Christopher S. Chen
    • 1
  1. 1.Department of BioengineeringUniversity of PennsylvaniaPhiladelphiaUSA

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