Measuring Cell–Cell Tugging Forces Using Bowtie-Patterned mPADs (Microarray Post Detectors)
Cells generate traction forces upon adhesion to the extracellular matrix as well as to neighboring cells. These forces are important for the growth and maintenance of adhesion structures such as focal adhesions and adherens junctions, and may play roles in tissue development. Here, we describe a method for measuring the tugging force transmitted across the cell–cell junction between two paired cells.
Key wordsCell–cell tugging force Cell–cell junctions Micropatterning mPADs Traction force
This work was supported by NIH grants HL73305 and through the RESBIO program (Integrated Technology Resource for Polymeric Biomaterials, NIH grant EB001046).
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