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Microencapsulation of Stem Cells to Study Cellular Interactions

  • Keith Moore
  • Adam Vandergriff
  • Jay D. Potts
Protocol
  • 2.6k Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 1066)

Abstract

Microencapsulation is a technique used in both controlled delivery of materials over time as well as preservation of these materials while delivery is occurring. The range of materials able to be encapsulated is variable, from drugs to living cells. The latter is described here. Electrospray microencapsulation applies a high-voltage field, through which a polymeric material is extruded. A gelling bath, comprising a cross-linking material, is used to create a stable hydrogel containing secondary substances intended for delivery. Control of extrusion parameters, such as flow rate and voltage, allows for specification of diameter and pore sizes of the microcapsules.

Key words

Electrospray Microencapsulation Bone marrow stromal cells Sodium alginate Transfection Electrohydrodynamics 

Notes

Acknowledgements

This work was supported by NIH grant HL086901 (JDP).

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Copyright information

© Springer Science+Business Media, New York 2013

Authors and Affiliations

  • Keith Moore
    • 1
  • Adam Vandergriff
    • 1
  • Jay D. Potts
    • 1
    • 2
  1. 1.Biomedical Engineering ProgramUniversity of South CarolinaColumbiaUSA
  2. 2.Department of Cell Biology and AnatomyUniversity of South Carolina School of MedicineColumbiaUSA

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