Microencapsulation is a technique used in both controlled delivery of materials over time as well as preservation of these materials while delivery is occurring. The range of materials able to be encapsulated is variable, from drugs to living cells. The latter is described here. Electrospray microencapsulation applies a high-voltage field, through which a polymeric material is extruded. A gelling bath, comprising a cross-linking material, is used to create a stable hydrogel containing secondary substances intended for delivery. Control of extrusion parameters, such as flow rate and voltage, allows for specification of diameter and pore sizes of the microcapsules.
Electrospray Microencapsulation Bone marrow stromal cells Sodium alginate Transfection Electrohydrodynamics
This is a preview of subscription content, log in to check access.
Springer Nature is developing a new tool to find and evaluate Protocols. Learn more
This work was supported by NIH grant HL086901 (JDP).
Chew S, Wen Y, Dzenis Y et al (2006) The role of electrospinning in the emerging field of nanomedicine. Curr Pharm Des 12:4751–4770PubMedCrossRefGoogle Scholar
Pham Q, Sharma U, Mikos A (2006) Electrospinning of polymeric nanofibers for tissue engineering applications: a review. Tissue Eng 12:1197–1211PubMedCrossRefGoogle Scholar
Chakraborty S, Liao I, Adler A et al (2009) Electrohydrodynamics: a facile technique to fabricate drug delivery systems. Adv Drug Deliv Rev 61:1043–1054PubMedCrossRefGoogle Scholar
Lin J, Yu W, Liu X et al (2008) In vitro and in vivo characterization of alginate-chitosan-alginate artificial microcapsules for therapeutic oral delivery of live bacterial cells. J Biosci Bioeng 105:660–665PubMedCrossRefGoogle Scholar
Zhang W, Yang G, Zhang A et al (2010) Preferential vitrification of water in small alginate microcapsules significantly augments cell cryopreservation by vitrification. Biomed Microdevices 12:89–96PubMedCrossRefGoogle Scholar
Paul A, Shum-Tim D, Prakash S (2010) Investigation on PEG integrated alginate-chitosan microcapsules for myocardial therapy using marrow stem cells genetically modified by recombinant baculovirus. Cardiovasc Eng Technol 1:154–164CrossRefGoogle Scholar
Zhang WJ, Li BG, Zhang C et al (2008) Biocompatibility and membrane strength of C3H10T1/2 cell-loaded alginate-based microcapsules. Cryotherapy 10:90–97CrossRefGoogle Scholar
Gronthos S, Akintoye S, Wang CY et al (2006) Bone marrow stromal stem cells for tissue engineering. Periodontology 2000(41):188–195CrossRefGoogle Scholar
Jiang Y, Jahagirdar B, Reinhardt RL et al (2002) Pluripotency of mesenchymal stem cells derived from adult marrow. Nature 418:41–49PubMedCrossRefGoogle Scholar
Khatri M, O’Brien T, Sharma JM (2009) Isolation and differentiation of chicken mesenchymal stem cells from bone marrow. Stem Cells Dev 18:1485–1492PubMedCrossRefGoogle Scholar
Martin D, Cox N, Hathcock TL et al (2002) Isolation and characterization of multipotential mesenchymal stem cells from feline bone marrow. Exp Hematol 30:879–886PubMedCrossRefGoogle Scholar
Moore K, Amos J, Davis J et al (2012) Characterization of polymeric microcapsules containing a low molecular weight peptide for controlled release. Microsc Microanal 19: 213–226CrossRefGoogle Scholar