Methods and Applications of Serological Proteome Analysis
The study of the humoral response to infectious diseases and chronic diseases, such as cancer, is important for many reasons, including understanding the host response to disease, identification of protective antigens, vaccine development, and discovery of biomarkers for early diagnosis. During the past decade, proteomic approaches, such as serological proteome analysis (SERPA), have been used to identify the repertoire of immunoreactive proteins in various diseases. In this chapter, we provide an outline of the SERPA approach, using the analysis of sera from mice vaccinated with a live attenuated tularemia vaccine as an example.
Key wordsSERPA Serological proteome analysis Western blotting Immunoproteomics Comparative immunoproteomics Francisella Tularemia Live vaccine strain
This work was supported by Federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, Contract No. HHSN266200500041C and in part by the National Research Council, Canada. The authors thank Freyja Lynn, Drs Vicki Pierson, Kristin Debord, Patrick Sanz (National Institutes of Allergy and Infectious Diseases), Dr J. Wayne Conlan, Luc Tessier and Marianne Savicky (National Research Council, Canada), and Gretchen Stup (DynPort Vaccine Company) for their contributions throughout this work. The authors also thank Patricia Massel for method testing and critical reading of the manuscript.
- 9.Dennis DT, Inglesby TV, Henderson DA, Bartlett JG, Ascher MS, Eitzen E, Fine AD, Friedlander AM, Hauer J, Layton M, Lillibridge SR, McDade JE, Osterholm MT, O’Toole T, Parker G, Perl TM, Russell PK, Tonat K (2001) Tularemia as a biological weapon: medical and public health management. JAMA 285:2763–2773PubMedCrossRefGoogle Scholar
- 17.Huntley JF, Robertson GT, Norgard MV (2010) Method for the isolation of Francisella tularensis outer membranes. J Vis ExpGoogle Scholar