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Using Arabidopsis-Related Model Species (ARMS): Growth, Genetic Transformation, and Comparative Genomics

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Arabidopsis Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1062))

Abstract

The Arabidopsis-related model species (ARMS) Thellungiella salsuginea and Thellungiella parvula have generated broad interest in salt stress research. While general growth characteristics of these species are similar to Arabidopsis, some aspects of their life cycle require particular attention in order to obtain healthy plants, with a large production of seeds in a relatively short time. This chapter describes basic procedures for growth, maintenance, and Agrobacterium-mediated transformation of ARMS. Where appropriate, differences in requirements between Thellungiella spp. and Arabidopsis are highlighted, along with basic growth requirements of other less studied candidate model species. Current techniques for comparative genomics analysis between Arabidopsis and ARMS are also described in detail.

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Acknowledgements

Dong-Ha Oh thanks Eric Lyons for great help in setting up T. parvula sequences in CoGe database. Dong-Ha Oh was supported by World Class University Program (R32–10148) at Gyeongsang National University, Republic of Korea, and the Next-Generation BioGreen 21 Program (SSAC, PJ009495), Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. CNR-IGV Institute of Plant Genetics, Portici, Italy

    Giorgia Batelli & Hans J. Bohnert

  2. Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA

    Dong-Ha Oh, Maheshi Dassanayake & Hans J. Bohnert

  3. Division of Applied Science, Gyeongsang National University, Jinju, South Korea

    Dong-Ha Oh & Ray A. Bressan

  4. Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN, USA

    Matilde Paino D’Urzo, Jian-Kang Zhu & Ray A. Bressan

  5. Department of Agro-Environmental Sciences and Technology, University of Bologna, Bologna, Italy

    Francesco Orsini

  6. College of Science, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia

    Hans J. Bohnert & Ray A. Bressan

  7. Department of Agricultural Engineering and Agronomy, University of Naples Federico II, Portici, Italy

    Albino Maggio

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  1. Giorgia Batelli
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  2. Dong-Ha Oh
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  3. Matilde Paino D’Urzo
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  4. Francesco Orsini
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  5. Maheshi Dassanayake
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  6. Jian-Kang Zhu
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  7. Hans J. Bohnert
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  8. Ray A. Bressan
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Editors and Affiliations

  1. CSIC Centro Nacional de BiotecnologĂ­a, Madrid, Spain

    Jose J. Sanchez-Serrano

  2. Depto. Biologia de Plantas, CSIC Madrid Centro de Investigaciones Biologicas, Madrid, Spain

    Julio Salinas

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Batelli, G. et al. (2014). Using Arabidopsis-Related Model Species (ARMS): Growth, Genetic Transformation, and Comparative Genomics. In: Sanchez-Serrano, J., Salinas, J. (eds) Arabidopsis Protocols. Methods in Molecular Biology, vol 1062. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-580-4_2

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  • DOI: https://doi.org/10.1007/978-1-62703-580-4_2

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