Abstract
The large scale sequencing of insertion element flanking sequences has revolutionized reverse genetics in plant research: Insertion mutants can now simply be identified in silico by BLAST searching the resulting flanking sequence databases. The development of next-generation sequencing technologies has further facilitated the creation of flanking sequence collections derived from entire mutant populations. Here we describe a highly efficient and widely applicable method that we developed to amplify, sequence, and identify dTph1 transposon flanking sequences from a library of 1000 Petunia W138 individuals simultaneously.
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References
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Vandenbussche, M., Zethof, J., Gerats, T. (2013). Massive Indexed Parallel Identification of Transposon Flanking Sequences. In: Peterson, T. (eds) Plant Transposable Elements. Methods in Molecular Biology, vol 1057. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-568-2_18
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DOI: https://doi.org/10.1007/978-1-62703-568-2_18
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-567-5
Online ISBN: 978-1-62703-568-2
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