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Biological Aging pp 1–10Cite as

Cell Senescence Culturing Methods

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1048))

Abstract

Development of therapeutic approaches that slow or ablate the adverse physiological and pathological changes associated with aging has been considered as an important goal for gerontological research. As cellular senescence is characterized as the basis for aging in organisms, culturing and subculturing of normal human diploid fibroblasts to mimic the in vivo aging processes have been developed as major methods to investigate molecular events involved in aging. It has been established that normal human diploid fibroblasts can proliferate in culture for only finite periods of time. There are many ways to study aging in vitro. In this chapter, we will discuss some of the basic laboratory procedures for cell senescence culturing methods.

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Acknowledgments

This work was supported in part by grant from the NIH (CA129415) and the American Institute for Cancer Research.

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Authors and Affiliations

  1. Department of Biology, University of Alabama at Birmingham, Birmingham, AL, USA

    Huaping Chen, Yuanyuan Li & Trygve O. Tollefsbol

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  1. Huaping Chen
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  2. Yuanyuan Li
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  3. Trygve O. Tollefsbol
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Editors and Affiliations

  1. Dept. Biology, University of Alabama, Birmingham Dept. Biology, Birmingham, Alabama, USA

    Trygve O. Tollefsbol

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Chen, H., Li, Y., Tollefsbol, T.O. (2013). Cell Senescence Culturing Methods. In: Tollefsbol, T. (eds) Biological Aging. Methods in Molecular Biology, vol 1048. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-556-9_1

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  • DOI: https://doi.org/10.1007/978-1-62703-556-9_1

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-555-2

  • Online ISBN: 978-1-62703-556-9

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