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Use of Peptide Nucleic Acids (PNAs) for Genotyping by Solution and Surface Methods

  • Stefano Sforza
  • Tullia Tedeschi
  • Mariangela Bencivenni
  • Alessandro Tonelli
  • Roberto Corradini
  • Rosangela Marchelli
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1050)

Abstract

Peptide nucleic acids (PNAs) are synthetic oligonucleotide analogues based on a pseudopeptide backbone that bind complementary DNA or RNA with high affinity and specificity. In this chapter, three PNA-based genotyping assays are described: PCR clamping, fluorescence-based recognition, and microarray platform. The first two methods are performed in solution, while the microarray method uses a solid surface.

Key words

PNA PCR-clamping Fluorescence Microarrays Genotyping 

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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  • Stefano Sforza
    • 1
  • Tullia Tedeschi
    • 1
  • Mariangela Bencivenni
    • 1
  • Alessandro Tonelli
    • 1
  • Roberto Corradini
    • 2
  • Rosangela Marchelli
    • 2
  1. 1.Department of Food ScienceUniversity of ParmaParmaItaly
  2. 2.Department of ChemistryUniversity of ParmaParmaItaly

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