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In Vitro Model of Spontaneous Mouse OSE Transformation

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Ovarian Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1049))

Abstract

An in vitro syngeneic model of neoplastic progression of murine ovarian surface epithelial (MOSE) cells represents a valid and significant model that allows for investigations into early mechanisms that impact tumorigenesis. Importantly, MOSE cells representing different stages of neoplastic transformation can be implanted back into immunocompetent mice to investigate host microenvironmental interactions that impact peritoneal dissemination and suppress immune surveillance mechanisms. Here we describe the isolation of MOSE cells that undergo spontaneous transformation upon repeated passage in cell culture. We also provide detailed in vitro assays for 3-D culturing of MOSE cells for characterizing anchorage-independent and invasive growth properties of these cells. Cell lines derived from this model have provided numerous insights into genetic, epigenetic, and biomechanical changes associated with neoplastic progression, as well as the immune responses associated with peritoneal dissemination of ovarian cancer cells.

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Acknowledgements

This work was supported by NIH grant CA118846 (E.M.S., P.C.R.) and The Fralin Research Institute Initiative for Cancer Biology at Virginia Tech (P.C.R., E.M.S.).

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Authors and Affiliations

  1. Department of Biomedical Sciences and Pathobiology, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA

    Paul C. Roberts

  2. Department of Human Nutrition, Foods and Exercise, Virginia Tech, Blacksburg, VA, USA

    Eva M. Schmelz

Authors
  1. Paul C. Roberts
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  2. Eva M. Schmelz
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Editor information

Editors and Affiliations

  1. Group of Experimental Therapeutics, Federal State Institution N.N.Petrov Res Institute of Oncology, St. Petersburg, Russia

    Anastasia Malek

  2. Labs of Molecular Tumor Pathology & Func, Charité - Universitatsmedizin Berlin, Berlin, Germany

    Oleg Tchernitsa

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Roberts, P.C., Schmelz, E.M. (2013). In Vitro Model of Spontaneous Mouse OSE Transformation. In: Malek, A., Tchernitsa, O. (eds) Ovarian Cancer. Methods in Molecular Biology, vol 1049. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-547-7_30

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  • DOI: https://doi.org/10.1007/978-1-62703-547-7_30

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-546-0

  • Online ISBN: 978-1-62703-547-7

  • eBook Packages: Springer Protocols

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