Skip to main content
SpringerLink
Log in

Assessment of Resistance to Anoikis in Ovarian Cancer

  • Protocol
  • First Online:
Ovarian Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1049))

Abstract

Anoikis, a form of programmed cell death that occurs due to cell detachment from the extracellular matrix, is a critical mechanism in preventing ectopic cell growth. Acquisition of resistance to anoikis is a prerequisite for epithelial ovarian cancer cells to survive in ascitic fluids before forming metastatic foci. Here we describe a colorimetric method for monitoring the resistance of anoikis of ovarian cancer cells in vitro.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Frisch SM, Francis H (1994) Disruption of epithelial cell-matrix interactions induces apoptosis. J Cell Biol 124:619–626

    Article  PubMed  CAS  Google Scholar 

  2. Simpson CD, Anyiwe K, Schimmer AD (2008) Anoikis resistance and tumor metastasis. Cancer Lett 272:177–185

    Article  PubMed  CAS  Google Scholar 

  3. Lee YS et al (2008) The cytoplasmic deacetylase HDAC6 is required for efficient oncogenic tumorigenesis. Cancer Res 68:7561–7569

    Article  PubMed  CAS  Google Scholar 

  4. Frankel A et al (2001) Induction of anoikis and suppression of human ovarian tumor growth in vivo by down-regulation of Bcl–X(L). Cancer Res 61:4837–4841

    PubMed  CAS  Google Scholar 

  5. Douma S et al (2004) Suppression of anoikis and induction of metastasis by the neurotrophic receptor TrkB. Nature 430:1034–1039

    Article  PubMed  CAS  Google Scholar 

  6. Cheng KW et al (2004) The RAB25 small GTPase determines aggressiveness of ovarian and breast cancers. Nat Med 10:1251–1256

    Article  PubMed  CAS  Google Scholar 

  7. Sood AK et al (2010) Adrenergic modulation of focal adhesion kinase protects human ovarian cancer cells from anoikis. J Clin Invest 120:1515–1523

    Article  PubMed  CAS  Google Scholar 

  8. He X et al (2010) Downregulation of HtrA1 promotes resistance to anoikis and peritoneal dissemination of ovarian cancer cells. Cancer Res 70:3109–3118

    Article  PubMed  CAS  Google Scholar 

  9. Yu X et al (2008) Suppression of anoikis by the neurotrophic receptor TrkB in human ovarian cancer. Cancer Sci 99:543–552

    Article  PubMed  CAS  Google Scholar 

  10. Mailleux AA et al (2007) BIM regulates apoptosis during mammary ductal morphogenesis, and its absence reveals alternative cell death mechanisms. Dev Cell 12:221–234

    Article  PubMed  CAS  Google Scholar 

  11. Gottlieb E (2009) Cancer: the fat and the furious. Nature 461:44–45

    Article  PubMed  CAS  Google Scholar 

  12. Schafer ZT et al (2009) Antioxidant and oncogene rescue of metabolic defects caused by loss of matrix attachment. Nature 461:109–113

    Article  PubMed  CAS  Google Scholar 

  13. Shen S et al (2011) Association and dissociation of autophagy, apoptosis and necrosis by systematic chemical study. Oncogene 30:4544–4556

    Article  PubMed  CAS  Google Scholar 

  14. Horbinski C, Mojesky C, Kyprianou N (2010) Live free or die: tales of homeless (cells) in cancer. Am J Pathol 177:1044–1052

    Article  PubMed  CAS  Google Scholar 

  15. Kenific CM, Thorburn A, Debnath J (2010) Autophagy and metastasis: another double-edged sword. Curr Opin Cell Biol 22:241–245

    Article  PubMed  CAS  Google Scholar 

  16. Coates JM, Galante JM, Bold RJ (2010) Cancer therapy beyond apoptosis: autophagy and anoikis as mechanisms of cell death. J Surg Res 164:301–308

    Article  PubMed  Google Scholar 

  17. Sher I et al (2009) Autocrine VEGF-A/KDR loop protects epithelial ovarian carcinoma cells from anoikis. Int J Cancer 124:553–561

    Article  PubMed  CAS  Google Scholar 

  18. Tang MK et al (2010) c-Met overexpression contributes to the acquired apoptotic resistance of nonadherent ovarian cancer cells through a cross talk mediated by phosphatidylinositol 3-kinase and extracellular signal-regulated kinase 1/2. Neoplasia 12:128–138

    PubMed  CAS  Google Scholar 

  19. Frisch SM, Francis H (1994 Feb) Disruption of epithelial cell-matrix interactions induces apoptosis. J Cell Biol 124(4):619–626

    Article  PubMed  CAS  Google Scholar 

  20. Fung C, Lock R, Gao S, Salas E, Debnath J (2008 Mar) Induction of autophagy during extracellular matrix detachment promotes cell survival. Mol Biol Cell 19(3):797–806

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by NIH Training Grant CA148073-02 (to X.H.), the Ovarian Cancer Research Fund PEO/MC/01.08 (to X. H.), National Cancer Institute grant CA12340 (to V.S. and J.C.), and the Mayo Clinic College of Medicine (to V.S.).

Author information

Authors and Affiliations

  1. Mayo Clinic College of Medicine, Rochester, MN, USA

    Xiaoping He, Jeremy Chien & Viji Shridhar

Authors
  1. Xiaoping He
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jeremy Chien
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Viji Shridhar
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Group of Experimental Therapeutics, Federal State Institution N.N.Petrov Res Institute of Oncology, St. Petersburg, Russia

    Anastasia Malek

  2. Labs of Molecular Tumor Pathology & Func, Charité - Universitatsmedizin Berlin, Berlin, Germany

    Oleg Tchernitsa

Rights and permissions

Reprints and permissions

Copyright information

© 2013 Springer Science+Business Media, New York

About this protocol

Cite this protocol

He, X., Chien, J., Shridhar, V. (2013). Assessment of Resistance to Anoikis in Ovarian Cancer. In: Malek, A., Tchernitsa, O. (eds) Ovarian Cancer. Methods in Molecular Biology, vol 1049. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-547-7_26

Download citation

  • DOI: https://doi.org/10.1007/978-1-62703-547-7_26

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-546-0

  • Online ISBN: 978-1-62703-547-7

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation