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Ovarian Cancer Stem Cells Enrichment

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Ovarian Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1049))

Abstract

The concept of cancer stem cells (CSCs) provides a new paradigm for understanding cancer biology. Cancer stem cells are defined as a minority of cancer cells with stem cell properties responsible for maintenance and growth of tumors. The targeting of CSCs is a potential therapeutic strategy to combat ovarian cancer. Ovarian epithelial cancer cells cultured in serum-free medium can form sphere cells. These sphere cells may be enriched for cancer stem cells (CSCs). The isolation of sphere cells from solid tumors is an important technique in studying cancer cell biology. Here we describe the isolation of sphere cells from primary ovarian cancer tissue, ascites fluid, and the cancer cell line SKOV3 with stem cell selection medium.

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Acknowledgments

This work was supported by the Science and Technology Commission of Shanghai Municipality 2007 Shanghai Pujiang project (to D.L. 07pj14090), the Science and Technology Commission of Shanghai Municipality 2009 YIXUEYINGDAO project (to D.L. 09411968300), and the Key Project Fund of Shanghai Municipal Health Bureau (to D.L. 2010011), Shanghai, China.

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Authors and Affiliations

  1. The International peace maternity and child health hospital, School of medicine, Shanghai Jiaotong University, Shanghai, China

    Lijuan Yang & Dongmei Lai

Authors
  1. Lijuan Yang
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  2. Dongmei Lai
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Editors and Affiliations

  1. Group of Experimental Therapeutics, Federal State Institution N.N.Petrov Res Institute of Oncology, St. Petersburg, Russia

    Anastasia Malek

  2. Labs of Molecular Tumor Pathology & Func, Charité - Universitatsmedizin Berlin, Berlin, Germany

    Oleg Tchernitsa

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© 2013 Springer Science+Business Media, New York

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Yang, L., Lai, D. (2013). Ovarian Cancer Stem Cells Enrichment. In: Malek, A., Tchernitsa, O. (eds) Ovarian Cancer. Methods in Molecular Biology, vol 1049. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-547-7_25

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  • DOI: https://doi.org/10.1007/978-1-62703-547-7_25

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-546-0

  • Online ISBN: 978-1-62703-547-7

  • eBook Packages: Springer Protocols

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