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Synthesis of O-Glycopeptides and Construction of Glycopeptide Microarrays

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Peptide Synthesis and Applications

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1047))

Abstract

O-Glycosylation of proteins is an important modification which affects biological function and immunity. In this chapter, we provide protocols for efficient solid-phase O-glycopeptide synthesis (SPGPS) and protocols for the construction of glycopeptide microarray chips for screening applications. This will be exemplified for mucin-type glycopeptides and the construction of glycopeptide microarrays. To this end, the protocols provided are particularly suited for small-scale robotic parallel synthesis. N-Terminal amine capping of deletion peptides during synthesis stands out as vital to this strategy. It allows for direct on-slide enrichment of the full-length target product and thereby bypasses tedious isolation and purification procedures.

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Acknowledgements

This work was supported by The Benzon Foundation, The Danish Agency for Science, Technology and Innovation (FTP), EU FP7/2007-2013-EuroGlycoArrays 215536, EU FP7-GlycoBioM, and University of Copenhagen Programme of Excellence.

Author information

Authors and Affiliations

  1. Department of Chemistry, Faculty of Sciences, University of Copenhagen, Copenhagen, Denmark

    Ola Blixt

  2. Novo Nordisk A/S, Måløv, Denmark

    Emiliano Cló

Authors
  1. Ola Blixt
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  2. Emiliano Cló
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Editor information

Editors and Affiliations

  1. IGM, Faculty of Sciences, University of Copenhagen, Frederiksberg, Denmark

    Knud J. Jensen

  2. IGM, Faculty of Science, University of Copenhagen, Glostrup, Denmark

    Pernille Tofteng Shelton

  3. IGM, Faculty of Life Sciences, University of Copenhagen, Hørsholm, Denmark

    Søren L. Pedersen

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Blixt, O., Cló, E. (2013). Synthesis of O-Glycopeptides and Construction of Glycopeptide Microarrays. In: Jensen, K., Tofteng Shelton, P., Pedersen, S. (eds) Peptide Synthesis and Applications. Methods in Molecular Biology, vol 1047. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-544-6_14

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  • DOI: https://doi.org/10.1007/978-1-62703-544-6_14

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-543-9

  • Online ISBN: 978-1-62703-544-6

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