Abstract
Many proteins that reside in the cytoplasm bind directly to cell membranes and play roles in signaling, adhesion, metabolism, cell structure, and cell motility. Several of these membrane-binding proteins, especially cytoskeletal proteins, have mechanical functions that result in the transmission of forces to the plasma membrane and organelle-membranes. Despite the importance of these interactions, remarkably little is known about the mechanical properties of the bonds between membranes and proteins. In this chapter, we describe a single-molecule, optical-trapping method for the measurement of protein–membrane adhesion forces and force-dependent attachment-lifetimes.
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Acknowledgment
This work was supported by a grant from the NIH (GM057247) to E.M.O.
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Pyrpassopoulos, S., Shuman, H., Ostap, E.M. (2013). Method for Measuring Single-Molecule Adhesion Forces and Attachment Lifetimes of Protein–Membrane Interactions. In: Coutts, A. (eds) Adhesion Protein Protocols. Methods in Molecular Biology, vol 1046. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-538-5_24
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DOI: https://doi.org/10.1007/978-1-62703-538-5_24
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Publisher Name: Humana Press, Totowa, NJ
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