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Loop-Mediated Isothermal Amplification Method for a Differential Identification of Human Taenia Tapeworms

  • Yasuhito Sako
  • Agathe Nkouawa
  • Tetsuya Yanagida
  • Akira Ito
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1039)

Abstract

Loop-mediated isothermal amplification (LAMP), which employs a Bst DNA polymerase with strand-displacement activity and four primers (two inner primers and two outer primers) recognizing six distinct regions on the target DNA, is a highly sensitive, specific, simple, and rapid nucleotide amplification method. Moreover, because the Bst DNA polymerase resists much DNA polymerase inhibitors present in biological specimens, the LAMP method is suitable for the detection of infectious agents from clinical material such as fecal samples. Here, we describe the LAMP method which can differentially detect and identify human Taenia tapeworms, Taenia solium, T. saginata, and T. asiatica, using DNA specimens prepared from parasite tissue and human fecal sample.

Key words

Loop-mediated isothermal amplification Taenia solium T. saginata T. asiatica Differential detection Proglottid Fecal sample 

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Copyright information

© Springer Science+Business Media, New York 2013

Authors and Affiliations

  • Yasuhito Sako
    • 1
  • Agathe Nkouawa
    • 1
  • Tetsuya Yanagida
    • 1
  • Akira Ito
    • 1
  1. 1.Department of ParasitologyAsahikawa Medical UniversityAsahikawaJapan

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