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Detection of SNP-Containing Human DNA Sequences Using a Split Sensor with a Universal Molecular Beacon Reporter

  • Yulia V. Gerasimova
  • Jack Ballantyne
  • Dmitry M. Kolpashchikov
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1039)

Abstract

Hybridization-based techniques have been extensively employed for the analysis of specific DNA/RNA sequences. Herein, we describe highly specific inexpensive smart hybridization-based sensor that takes advantage of a universal molecular beacon probe as a fluorescent reporter. The sensor has a straightforward design, and demonstrates improved selectivity and specificity of nucleic acid recognition. It is cost-efficient since it utilizes the same molecular beacon probe for the analysis of many nucleic acid sequences.

Key words

Molecular beacon probe High specificity DNA junctions Genotyping Forensic SNPs 

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Copyright information

© Springer Science+Business Media, New York 2013

Authors and Affiliations

  • Yulia V. Gerasimova
    • 1
  • Jack Ballantyne
    • 2
    • 1
  • Dmitry M. Kolpashchikov
    • 1
  1. 1.Department of ChemistryUniversity of Central FloridaOrlandoUSA
  2. 2.National Center for Forensic ScienceOrlandoUSA

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