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Application of Caged Fluorescent Nucleotides to Live-Cell RNA Imaging

  • Akimitsu Okamoto
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1039)

Abstract

A caged fluorescent nucleic acid probe, which contains a nucleotide modified with one photolabile nitrobenzyl unit and two hybridization-sensitive thiazole orange units, has been synthesized for area-specific fluorescence imaging of RNA in a cell. The probe emits very weak fluorescence regardless of the presence of the complementary RNA, whereas it shows hybridization-sensitive fluorescence emission after photoirradiation for uncaging. Such probes are generated via several chemical synthetic steps and are applicable to area-specific RNA imaging in a cell. Only probes that exist in the defined irradiation area are activated via uncaging irradiation.

Key words

Caged nucleic acid Photolysis Fluorescent probe RNA imaging 

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Copyright information

© Springer Science+Business Media, New York 2013

Authors and Affiliations

  • Akimitsu Okamoto
    • 1
  1. 1.The Research Center for Advanced Science and Technology (RCAST)The University of TokyoTokyoJapan

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