High-Throughput Functional MicroRNA Profiling Using Recombinant AAV-Based MicroRNA Sensor Arrays
There is a lack of methods for high-throughput functional microRNA (miRNA) profiling. In this chapter, we describe a recombinant adeno-associated virus-based miRNA sensor array (miRNA Asensor array), which is able to profile functional miRNAs in cultured cells. The preparation of an miRNA Asensor array and its usage are discussed.
Key wordsmiRNA miRNA activity high-throughput miRNA Asensor array
This work was supported by a grant from the National High Technology Research and Development Program of China (No. 2012AA020810).
- 4.Chen B, Zhang B, Luo H, Yuan J, Skogerbø G, Chen R (2012) Distinct microRNA subcellular size and expression patterns in human cancer cells. Int J Cell Biol 2012, article ID 672462Google Scholar
- 17.Blaho JA, Morton ER, Yedowitz JC (2005) Herpes simplex virus: propagation, quantification, and storage. Curr Protoc Microbiol, Chapter 14, Unit 14E.1
- 19.Haberman RA, Kroner-Lux G, McCown TJ, Samulski RJ (1999) Production of recombinant adeno-associated viral vectors and use in in vitro and in vivo administration. Curr Protoc Neurosci (Suppl 9), Unit 4.17Google Scholar