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Electrochemiluminescence Detection of c-Myc mRNA in Breast Cancer Cells on a Wireless Bipolar Electrode

  • Mei-Sheng Wu
  • Guang-sheng Qian
  • Jing-Juan Xu
  • Hong-Yuan Chen
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1039)

Abstract

Electrochemiluminescence (ECL) on bipolar electrode (BPE) is a sensitive, portable, and low-cost approach which has been employed to detect DNA and proteins. Here, we develop an ultrasensitive method for intracellular mRNA assay based on mRNA-mediated reporter DNA liberation and Ru(bpy)3 2+-conjugated silica nanoparticles (RuSi@Ru(bpy)3 2+) tag-based signal amplification.

Key words

Bipolar electrode (BPE) Electrochemiluminescence (ECL) Tumor cells mRNA Quantum dots Signal amplification 

Notes

Acknowledgment

This work was supported by the 973 Program (Grant 2012CB932600), the National Natural Science Foundation (Grants 21025522 and 20890020) of China.

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Copyright information

© Springer Science+Business Media, New York 2013

Authors and Affiliations

  • Mei-Sheng Wu
    • 1
  • Guang-sheng Qian
    • 1
  • Jing-Juan Xu
    • 1
  • Hong-Yuan Chen
    • 1
  1. 1.State Key Laboratory of Analytical Chemistry for Life ScienceSchool of Chemistry and Chemical Engineering, Nanjing UniversityNanjingChina

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