Abstract
The detection of nucleic acid is of central importance for the diagnosis of genetic diseases, infectious agents, and biowarfare agents. Traditional strategies and technologies for nucleic acid detection are time-consuming and labor-intensive. Recently, isothermal strand-displacement reaction-based lateral flow biosensors have attracted a great deal of research interest because they are sensitive, simple, fast, and easy to use. Here, we describe a lateral flow biosensor based on isothermal strand-displacement polymerase reaction and gold nanoparticles for the visual detection of nucleic acid.
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References
Sampath R, Hall TA, Massire C, Li F, Blyn LB, Eshoo MW et al (2007) Rapid identification of emerging infectious agents using PCR and electrospray ionization mass spectrometry. Ann N Y Acad Sci 1102:109–120
Park SJ, Taton TA, Mirkin CA (2002) Array-based electrical detection of DNA with nanoparticle probes. Science 295:1503–1506
Elkady A, Tanaka Y, Kurbanov F, Sugauchi F, Sugiyama M, Khan A et al (2010) Performance of two real-time RT-PCR assays for quantitation of hepatitis C virus RNA: evaluation on HCV genotypes 1–4. J Med Virol 82:1878–1888
Liu RH, Yang JN, Lenigk R, Bonanno J, Grodzinski P (2004) Self-contained, fully integrated biochip for sample preparation, polymerase chain reaction amplification, and DNA microarray detection. Anal Chem 76:1824–1831
He L, Musick MD, Nicewarner SR, Salinas FG, Benkovic SJ, Natan MJ et al (2000) Colloidal Au-enhanced surface plasmon resonance for ultrasensitive detection of DNA hybridization. J Am Chem Soc 122:9071–9077
Sipova H, Zhang SL, Dudley AM, Galas D, Wang K, Homola J (2010) Surface plasmon resonance biosensor for rapid label-free detection of microribonucleic acid at subfemtomole level. Anal Chem 82:10110–10115
Guo QP, Yang XH, Wang KM, Tan W, Li W, Tang H et al (2009) Sensitive fluorescence detection of nucleic acids based on isothermal circular strand-displacement polymerization reaction. Nucleic Acids Res 37:e30
Shi C, Ge Y, Gu H, Ma C (2011) Highly sensitive chemiluminescent point mutation detection by circular strand-displacement amplification reaction. Biosens Bioelectron 26:4697–4701
He YQ, Zeng K, Zhang XB, Gurung AS, Baloda M, Xu H et al (2010) Ultrasensitive electrochemical detection of nucleic acid based on the isothermal strand-displacement polymerase reaction and enzyme dual amplification. Electrochem Commun 12:985–988
Fang ZY, Ge CC, Zhang WJ, Lie PC, Zeng LW (2011) A lateral flow biosensor for rapid detection of DNA-binding protein c-jun. Biosens Bioelectron 27:192–196
Chen J, Fang Z, Lie P, Zeng L (2012) Computational lateral flow biosensor for proteins and small molecules: a new class of strip logic gates. Anal Chem 84:6321–6325
Liu GD, Mao X, Phillips JA, Xu H, Tan WH, Zeng LW (2009) Aptamer-nanoparticle strip biosensor for sensitive detection of cancer cells. Anal Chem 81:10013–10018
Fang Z, Huang J, Lie P, Xiao Z, Ouyang C, Wu Q et al (2010) Lateral flow nucleic acid biosensor for Cu2+ detection in aqueous solution with high sensitivity and selectivity. Chem Commun 46:9043–9045
Acknowledgments
This work was financially supported by The Foundation of Special Research Program of Science and Technology Bureau of Guangzhou (No. 2008Z1-E581, No. 2009Ss-P030), Chinese Academy of Science (KSCX2-YW-R-164), and Department of Education of Guangdong Province (GXZD0901).
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Zeng, L., Lie, P., Fang, Z., Xiao, Z. (2013). Lateral Flow Biosensors for the Detection of Nucleic Acid. In: Kolpashchikov, D., Gerasimova, Y. (eds) Nucleic Acid Detection. Methods in Molecular Biology, vol 1039. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-535-4_14
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DOI: https://doi.org/10.1007/978-1-62703-535-4_14
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-62703-535-4
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