Lateral Flow Biosensors for the Detection of Nucleic Acid

  • Lingwen Zeng
  • Puchang Lie
  • Zhiyuan Fang
  • Zhuo Xiao
Part of the Methods in Molecular Biology book series (MIMB, volume 1039)


The detection of nucleic acid is of central importance for the diagnosis of genetic diseases, infectious agents, and biowarfare agents. Traditional strategies and technologies for nucleic acid detection are time-consuming and labor-intensive. Recently, isothermal strand-displacement reaction-based lateral flow biosensors have attracted a great deal of research interest because they are sensitive, simple, fast, and easy to use. Here, we describe a lateral flow biosensor based on isothermal strand-displacement polymerase reaction and gold nanoparticles for the visual detection of nucleic acid.

Key words

Lateral flow biosensor Nucleic acid Gold nanoparticle 



This work was financially supported by The Foundation of Special Research Program of Science and Technology Bureau of Guangzhou (No. 2008Z1-E581, No. 2009Ss-P030), Chinese Academy of Science (KSCX2-YW-R-164), and Department of Education of Guangdong Province (GXZD0901).


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Copyright information

© Springer Science+Business Media, New York 2013

Authors and Affiliations

  • Lingwen Zeng
    • 1
  • Puchang Lie
    • 1
  • Zhiyuan Fang
    • 1
  • Zhuo Xiao
    • 1
  1. 1.Key Laboratory of Regenerative BiologySouth China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesGuangzhouChina

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