Abstract
In forensic casework analysis it is sometimes necessary to obtain genetic profiles from increasingly smaller amounts of biological material left behind by persons involved in criminal offenses. The ability to obtain profiles from trace biological evidence is routinely demonstrated with the so-called touch DNA evidence (generally perceived to be the result of DNA obtained from shed skin cells transferred from donor to an object or a person during physical contact). The current method of recovery of trace DNA employs cotton swabs or adhesive tape to sample an area of interest. While of practical utility, such a “blind-swabbing” approach will necessarily co-sample cellular material from the different individuals whose cells are present on the item, even if the individuals’ cells are located in geographically distinct locations on the item. Thus some of the DNA mixtures encountered in such touch DNA samples are artificially created by the swabbing itself. Therefore, a specialized approach for the isolation of single or few cells from “touch DNA evidence” is necessary in order to improve the analysis and interpretation of profiles recovered from these samples. Here, we describe an optimized and efficient removal strategy for the collection of cellular microparticles present in “touch DNA” samples, as well as enhanced amplification strategies to permit the recovery of short tandem repeat profiles of the donor(s) of the recovered microparticles.
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References
Bright JA, Petricevic SF (2004) Recovery of trace DNA and its application to DNA profiling of shoe insoles. Forensic Sci Int 145:7–12
Goray M, Eken E, Mitchell RJ, van Oorschot RA (2010) Secondary DNA transfer of biological substances under varying test conditions. Forensic Sci Int Genet 4:62–67
Hall D, Fairley M (2004) A single approach to the recovery of DNA and firearm discharge residue evidence. Sci Justice 44:15–19
Pang BC, Cheung BK (2007) Double swab technique for collecting touched evidence. Leg Med (Tokyo) 9:181–184
Petricevic SF, Bright JA, Cockerton SL (2006) DNA profiling of trace DNA recovered from bedding. Forensic Sci Int 159:21–26
Wickenheiser RA (2002) Trace DNA: a review, discussion of theory, and application of the transfer of trace quantities of DNA through skin contact. J Forensic Sci 47:442–450
Balogh MK, Burger J, Bender K, Schneider PM (2003) STR genotyping and mtDNA sequencing of latent fingerprint on paper. Forensic Sci Int 137:188–195
Barbaro A, Cormaci P, Teatino A, La Marca A, Barbaro A (2004) Anonymous letters? DNA and fingerprints technologies combined to solve a case. Forensic Sci Int 146:S133–S134
Castello A, Alvarez M, Verdu F (2004) DNA from a computer keyboard. Forensic Sci Commun 6. http://www.fbi.gov/about-us/lab/forensic-science-communications/fsc/july2004/case/2004_03_case01.htm
Horsman-Hall KM, Orihuela Y, Karczynski SL, Davis AL, Ban JD, Greenspoon SA (2009) Development of STR profiles from firearms and fired cartridge cases. Forensic Sci Int Genet 3:242–250
van Oorschot RA, Jones MK (1997) DNA fingerprints from fingerprints. Nature 387:767
Port N, Bowyer V, Graham E, Batuwangala MS, Rutty GN (2005) How long does it take a static speaking individual to contaminate the immediate environment? Forensic Sci Med Pathol 2:157–163
Alessandrini F, Cecati M, Pesaresi M, Turchi C, Carle F, Tagliabracci A (2003) Fingerprints as evidence for a genetic profile: morphological study on fingerprints and analysis of exogenous and individual factors affecting DNA typing. J Forensic Sci 48:586–592
Kita T, Yamaguchi H, Yokoyama M, Tanaka T, Tanaka N (2008) Morphological study of fragmented DNA on touched objects. Forensic Sci Int Genet 3:32–36
Raymond JJ, van Oorschot RA, Gunn PR, Walsh SJ, Roux C (2009) Trace evidence characteristics of DNA: a preliminary investigation of the persistence of DNA at crime scenes. Forensic Sci Int Genet 4:26–33
Kelley-Primozic K (2008) Sperm recovery. Evidence Technol Mag 6:18–19
Di MD, Giuffre G, Staiti N, Simone A, Le Donne M, Saravo L (2004) Single sperm cell isolation by laser microdissection. Forensic Sci Int 146:S151–S153
Hubert R, Weber JL, Schmitt K, Zhang L, Arnheim N (1992) A new source of polymorphic DNA markers for sperm typing: analysis of microsatellite repeats in single cells. Am J Hum Genet 51:985–991
Ensenberger MG, Thompson J, Hill B, Homick K, Kearney V, Mayntz-Press KA et al (2010) Developmental validation of the PowerPlex 16 HS System: an improved 16-locus fluorescent STR multiplex. Forensic Sci Int Genet 4:257–264
Wang DY, Chang CW, Lagace RE, Calandro LM, Hennessy LK (2012) Developmental validation of the AmpFlSTR(R) Identifiler(R) Plus PCR Amplification Kit: an established multiplex assay with improved performance. J Forensic Sci 57:453–465
Darmon M, Blumenberg M (eds) (1993) Molecular biology of the skin—the Keratinocyte. Academic, San Diego, CA
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Hanson, E.K., Ballantyne, J. (2013). “Getting Blood from a Stone”: Ultrasensitive Forensic DNA Profiling of Microscopic Bio-Particles Recovered from “Touch DNA” Evidence. In: Kolpashchikov, D., Gerasimova, Y. (eds) Nucleic Acid Detection. Methods in Molecular Biology, vol 1039. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-535-4_1
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DOI: https://doi.org/10.1007/978-1-62703-535-4_1
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Publisher Name: Humana Press, Totowa, NJ
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