Abstract
The unscheduled DNA synthesis test is a short-term genotoxicity assay that allows identification of substances that induce DNA repair in liver cells of treated animals. This endpoint can be assessed by determining the uptake of radiolabelled nucleotide after excision-repair in cells that are not undergoing scheduled (S-phase) DNA synthesis. Here we describe the protocol for performing this well-known primary DNA damage test following the OECD guideline No. 486, EC, and published international recommendations.
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References
Mirsalis J, Butterworth B (1980) Detection of unscheduled DNA synthesis in hepatocytes isolated from rats treated with genotoxic agents: an in vivo/in vitro assay for potential carcinogens and mutagens. Carcinogenesis 1:621–625
Ashby J, Lefevre PA, Burlinson B et al (1985) An assessment of the in vivo rat hepatocyte DNA repair assay. Mutat Res 156:1–18
Kennelly JC, Waters R, Ashby J et al (1993) In vivo rat liver UDS assay. Supplementary. In: Kirkland DJ, Fox M (eds.) Mutagenicity tests UKEMS recommended procedures. Cambridge University Press, pp 52–77
OECD (1997) Guideline for The Testing of Chemicals – Unscheduled DNA synthesis (UDS) test with mammalian liver cells in vivo – Guideline no. 486,.
EC – (2008) Commission Regulation No. 440/2008 – B.39. Unscheduled DNA synthesis (UDS) test with mammalian liver cells in vivo – Official Journal No. L 142
Nesslany F, Parent-Massin D, Marzin D (2010) Risk assessment of consumption of methylchavicol and tarragon: the genotoxic potential in vivo and in vitro. Mutat Res 696(1):1–9
Gaudin J, Le Hegarat L, Nesslany F et al (2009) In vivo genotoxic potential of microcystin-LR: a cyanobacterial toxin, investigated both by the unscheduled DNA synthesis (UDS) and the comet assays after intravenous administration. Environ Toxicol 24(2):200–209
Garry S, Nesslany F, el Aliouat M et al (2003) Potent genotoxic activity of benzo[a]pyrene coated onto hematite measured by unscheduled DNA synthesis in vivo in the rat. Mutagenesis 18(5):449–455
Seglen PO (1976) Preparation of isolated rat liver cells. Methods Cell Biol 13:29–83
Williams GM (1977) Detection of chemical carcinogens by unscheduled DNA synthesis in rat liver primary cell cultures. Cancer Res 37:1845–1851
Fautz R, Hussain B, Efstathiou E et al (1993) Assessment of the relation between the initial viability and the attachment of freshly isolated rat hepatocytes used for the in vivo/in vitro dna repair assay (UDS). Mutat Res 291:21–27
Brambilla G, Martelli A (1992) Grain counting in the in vitro hepatocyte DNA–repair assay. Mutat Res 272(1):9–15
Madle S, Dean SW, Andrae U et al (1994) Recommendations for the performance of UDS tests in vitro and in vivo. Mutat Res 312:263–285
Fielder RJ, Allen JA, Boobis AR et al (1993) Report of the BTS/UKEMS Working Group on dose setting in in vivo mutagenicity assays. Hum Exp Toxicol 12:189–198
Hamilton CM, Mirsalis JC (1987) Factors that affect the sensitivity of the in vivo–in vitro hepatocyte DNA repair assay in the male rat. Mutat Res 189(3):341–347
Butterworth BE, Ashby J, Bermudez E et al (1987) A protocol and guide for the in vivo rat hepatocyte DNA–repair assay. Mutat Res 189(2):123–133
No authors listed (1990) Omeprazole and genotoxicity. The Lancet. 335(8686):386
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Nesslany, F. (2013). Unscheduled DNA Synthesis (UDS) Test with Mammalian Liver Cells In Vivo. In: Dhawan, A., Bajpayee, M. (eds) Genotoxicity Assessment. Methods in Molecular Biology, vol 1044. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-529-3_20
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DOI: https://doi.org/10.1007/978-1-62703-529-3_20
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-62703-529-3
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