Abstract
Nucleotide-binding and leucine rich repeat domain-containing proteins (NLRs) are central to the formation of many inflammasome complexes. Several inflammasome forming NLR proteins are known to be ATPases, but the nucleotide binding specificity of many remains to be characterized. The oligomerization of NLR proteins and assembly of inflammasomes require the ATP (or other nucleotide) binding activity of the NLR proteins. Quantitative and qualitative studies of the nucleotide binding properties of these proteins are useful tools in studying the regulation of inflammasome activity, and are outlined in this Chapter.
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Acknowledgment
This work was supported by the National Institutes of Health through grant AI088255 and the Burroughs Wellcome Fund Career Award for Medical Scientists.
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Mo, J., Duncan, J.A. (2013). Assessing ATP Binding and Hydrolysis by NLR Proteins. In: De Nardo, C., Latz, E. (eds) The Inflammasome. Methods in Molecular Biology, vol 1040. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-523-1_12
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DOI: https://doi.org/10.1007/978-1-62703-523-1_12
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