Abstract
In vivo imaging with two-photon microscopy is becoming an indispensable technique to investigate cellular and subcellular phenomenon in living tissues including the central nervous system. This microscopy enables to image dynamics of molecules, morphology, and excitability with minimal invasion to tissues. Microglia are residual immune-responsive cells in the central nervous system and show highly dynamic response to the environmental alterations. Diverse roles of microglial functions in the intact and pathological brain are still largely unknown. In this chapter we describe the detailed method to image the dynamics of microglia in the mouse brain in vivo.
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Acknowledgments
S.O. is supported by Grants-in-Aid for Scientific Research (18200025, 20019013, 21220008, and 22650070), Global COE Program (Integrative Life Science Based on the Study of Biosignaling Mechanisms), and Strategic Research Program for Brain Sciences from MEXT Japan and by Takeda Science Foundation.
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Kondo, S., Okabe, S. (2013). In Vivo Two-Photon Microscopy of Microglia. In: Joseph, B., Venero, J. (eds) Microglia. Methods in Molecular Biology, vol 1041. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-520-0_28
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DOI: https://doi.org/10.1007/978-1-62703-520-0_28
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Publisher Name: Humana Press, Totowa, NJ
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