Skip to main content
Book cover

Cellular Cardiomyoplasty pp 207–212Cite as

Clinical Study Using Adipose-Derived Mesenchymal-Like Stem Cells in Acute Myocardial Infarction and Heart Failure

Part of the Methods in Molecular Biology book series (MIMB,volume 1036)

Abstract

Adipose tissue represents an abundant, accessible source of regenerative cells that can be easily obtained in sufficient amount for therapy. Adipose-derived regenerative cells (ADRC) are comprised of leukocytes, smooth muscles, endothelial cells, and mesenchymal stem cells. In contrast to bone-marrow-derived MSC, the abundance of adipose tissue in patients and the higher frequency per unit mass of regenerative cells allow for the isolation of cells in therapeutic meaningful amounts in less than 2h after donor tissue acquisition.

Harvest of adipose tissue can thus follow primary PCI, allowing efficient treatment within 24h. This obviates the need for extensive cell culturing in GMP clean room facilities and makes ADSCs a promising and practical autologous cell source. In the following chapter, we will describe the liposuction procedure for stem cell harvest, two cell delivery techniques, and pressure/volume loop analysis for the follow-up of our patients enrolled in the clinical studies.

Key words

  • ADSC
  • ADRC
  • Liposuction
  • Intracoronary infusion
  • Intramyocardial injection
  • Myocardial infarction
  • Heart failure
  • Stem cell therapy

This is a preview of subscription content, access via your institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • DOI: 10.1007/978-1-62703-511-8_16
  • Chapter length: 6 pages
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
eBook
USD   109.00
Price excludes VAT (USA)
  • ISBN: 978-1-62703-511-8
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD   139.00
Price excludes VAT (USA)
Hardcover Book
USD   149.99
Price excludes VAT (USA)
Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Caplan AI, Correa D (2011) The MSC: an Injury Drugstore. Cell Stem Cell 9:11–15. doi:10.1016/j.stem.2011.06.008

    CrossRef  PubMed  CAS  Google Scholar 

  2. Amado LC, Saliaris AP, Schuleri KH et al (2005) Cardiac repair with intramyocardial injection of allogeneic mesenchymal stem cells after myocardial infarction. Proc Natl Acad Sci U S A 102:11474–11479. doi:10.1073/pnas.0504388102

    CrossRef  PubMed  CAS  Google Scholar 

  3. Toma C (2002) Human mesenchymal stem cells differentiate to a cardiomyocyte phenotype in the adult murine heart. Circulation 105:93–98. doi:10.1161/hc0102.101442

    CrossRef  PubMed  Google Scholar 

  4. Gnecchi M, Zhang Z, Ni A, Dzau VJ (2008) Paracrine mechanisms in adult stem cell signaling and therapy. Circ Res 103:1204–1219. doi:10.1161/CIRCRESAHA.108.176826

    CrossRef  PubMed  CAS  Google Scholar 

  5. Clifford DM, Fisher SA, Brunskill SJ et al (2012) Stem cell treatment for acute myocardial infarction. Cochr Data System Rev (Online) 2:CD006536, doi: 10.1002/14651858.CD006536.pub3

  6. Friedenstein AJ, Chailakhjan RK, Lalykina KS (1970) The development of fibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells. Cell Tissue Kinet 3:393–403

    PubMed  CAS  Google Scholar 

  7. Pittenger MF (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284:143–147. doi:10.1126/science.284.5411.143

    CrossRef  PubMed  CAS  Google Scholar 

  8. Zuk PA, Zhu M, Ashjian P et al (2002) Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 13:4279–4295. doi:10.1091/mbc.E02-02-0105

    CrossRef  PubMed  CAS  Google Scholar 

  9. Caplan AI (2009) Why are MSCs therapeutic? New data: new insight. J Pathol 217:318–324. doi:10.1002/path.2469

    CrossRef  PubMed  CAS  Google Scholar 

  10. Fraser JK, Schreiber R, Strem B et al (2006) Plasticity of human adipose stem cells toward endothelial cells and cardiomyocytes. Nat Clin Pract Cardiovasc Med 3(Suppl 1):S33–S37. doi:10.1038/ncpcardio0444

    CrossRef  PubMed  CAS  Google Scholar 

  11. Houtgraaf JH, den Dekker WK, van Dalen BM et al (2012) First experience in humans using adipose tissue-derived regenerative cells in the treatment of patients with ST-segment elevation myocardial infarction. J Am Coll Cardiol 59:539–540. doi: 10.1016/j.jacc.2011.09.065

    CrossRef  PubMed  Google Scholar 

Download references

Acknowledgement

This work was supported by VIDI grant of Henricus J. Duckers, BioCardia Inc. (San Carlos), and Cytori Therapeutics Inc. (San Diego, California).

Author information

Authors and Affiliations

  1. Molecular Cardiology Laboratory & Intervention Cardiology, Thoraxcenter Rotterdam, Rotterdam, The Netherlands

    Ilia Alexander Panfilov, Renate de Jong & Shin-ichiro Takashima

  2. Department of Interventional Cardiology, Division of Cardiology & Pulmonology, University Medical Center Utrecht, Utrecht, The Netherlands

    Henricus J. Duckers

Authors
  1. Ilia Alexander Panfilov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Renate de Jong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shin-ichiro Takashima
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Henricus J. Duckers
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. , Department of Surgery, East Tennessee State University, Johnson City, 37614, Tennessee, USA

    Race L. Kao

Rights and permissions

Reprints and Permissions

Copyright information

© 2013 Springer Science+Business Media New York

About this protocol

Cite this protocol

Panfilov, I.A., de Jong, R., Takashima, Si., Duckers, H.J. (2013). Clinical Study Using Adipose-Derived Mesenchymal-Like Stem Cells in Acute Myocardial Infarction and Heart Failure. In: Kao, R. (eds) Cellular Cardiomyoplasty. Methods in Molecular Biology, vol 1036. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-511-8_16

Download citation

  • DOI: https://doi.org/10.1007/978-1-62703-511-8_16

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-510-1

  • Online ISBN: 978-1-62703-511-8

  • eBook Packages: Springer Protocols