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Visualization of Adult Stem Cells Within Their Niches Using the Drosophila Germline as a Model System

  • Annekatrin König
  • Halyna R. Shcherbata
Part of the Methods in Molecular Biology book series (MIMB, volume 1035)

Abstract

The germaria of the fruit fly Drosophila melanogaster present an excellent model to study germline stem cell–niche interactions. Two to three adult stem cells are surrounded by a number of somatic cells that form the niche. Here we describe how Drosophilae germaria can be dissected and specifically immuno-stained to allow for identification and analysis of both the adult stem cells and their somatic niche cells.

Key words

Drosophila Germarium Ovary Adult stem cells Stem cell niche Germline Ovarian soma Immunostaining 

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Annekatrin König
    • 1
  • Halyna R. Shcherbata
    • 1
  1. 1.Max Planck Research Group of Gene Expression and SignalingMax Planck Institute for Biophysical ChemistryGöttingenGermany

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