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Detection and Isolation of Human Disseminated Tumor Cells in the Murine Bone Marrow Stem Cell Niche

  • Yusuke Shiozawa
  • Russell S. Taichman
  • Evan T. Keller
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1035)

Abstract

The presence of disseminated tumor cells (DTCs) in the bone marrow is associated with poor prognosis of cancer patients. However, little is known about the biology of DTCs due to lack of relevant animal models. Here, we describe the methods for detecting and isolating human DTCs from the murine bone marrow niche by PCR using human Alu sequences and by fluorescence-activated cell sorting and immunohistochemistry using anti-HLA antibody. These strategies could be useful for exploring the biology of DTCs.

Key words

Disseminated tumor cells Bone marrow niche ALU Human leukocyte antigens PCR Flow cytometry Animal model Prostate cancer Immunohistochemistry 

Notes

Acknowledgment

We thank Elisabeth A. Pedersen, Aaron M. Havens, Jingcheng Wang (University of Michigan, Ann Arbor, Michigan, USA) for technical and logistical support. This work is directly supported by the National Cancer Institute (CA093900, E.T.K. and R.S.T., CA163124, Y.S. and R.S.T.), the Department of Defense (Y.S. and R.S.T.), and the Prostate Cancer Foundation (Y.S. and R.S.T.).

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Yusuke Shiozawa
    • 1
  • Russell S. Taichman
    • 1
  • Evan T. Keller
    • 2
  1. 1.Department of Periodontics and Oral MedicineUniversity of Michigan School of DentistryAnn ArborUSA
  2. 2.Departments of UrologyUniversity of Michigan Medical SchoolAnn ArborUSA

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