The Prospective Isolation of Viable, High Ploidy Megakaryocytes from Adult Murine Bone Marrow by Fluorescence Activated Cell Sorting
Mature megakaryocytes (MM) can be up to 65 μM in diameter and due to their size, viable and pure MM populations have been difficult to isolate in large numbers. Here in, we report a fluorescence activated cell sorting (FACS) method by which viable and pure populations of 8 N, 16 N, 32 N, and 64 N MM can be isolated from murine bone marrow (BM).
Key wordsMegakaryocytes Ploidy Bone marrow Microenvironment Niche
The authors thank Dani Cardozo for assistance with animal work, Michael Reitsma and Andrew Fryga for intellectual input and flow cytometric support. In addition, we also thank Kathryn Flanagan and Karen Clarke for flow cytometric support.
- 1.Shen Y, Nilsson SK (2012) Curr Opin Hematol 19(4):250–5Google Scholar
- 2.Heazlewood SY, Neaves RJ, Williams B, Haylock DN, Adams TE, Nilsson SK (in press) Stem Cell ResearchGoogle Scholar
- 15.Davies D (2007) Cell sorting by flow cytometry. In: Macey MG (ed) Flow cytometry: principles and applications, Humana Press, New Jersey, USA, pp 257–276.Google Scholar