The Prospective Isolation of Viable, High Ploidy Megakaryocytes from Adult Murine Bone Marrow by Fluorescence Activated Cell Sorting

  • Shen Y. Heazlewood
  • Brenda Williams
  • Melonie J. Storan
  • Susan K. Nilsson
Part of the Methods in Molecular Biology book series (MIMB, volume 1035)


Mature megakaryocytes (MM) can be up to 65 μM in diameter and due to their size, viable and pure MM populations have been difficult to isolate in large numbers. Here in, we report a fluorescence activated cell sorting (FACS) method by which viable and pure populations of 8 N, 16 N, 32 N, and 64 N MM can be isolated from murine bone marrow (BM).

Key words

Megakaryocytes Ploidy Bone marrow Microenvironment Niche 



The authors thank Dani Cardozo for assistance with animal work, Michael Reitsma and Andrew Fryga for intellectual input and flow cytometric support. In addition, we also thank Kathryn Flanagan and Karen Clarke for flow cytometric support.


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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Shen Y. Heazlewood
    • 1
  • Brenda Williams
    • 2
  • Melonie J. Storan
    • 2
  • Susan K. Nilsson
    • 2
    • 3
  1. 1.Materials Science and EngineeringCommonwealth Scientific and Industrial Research OrganizationMelbourneAustralia
  2. 2.Materials Science and EngineeringCommonwealth Scientific and Industrial Research OrganizationMelbourneAustralia
  3. 3.Department of Anatomy and Developmental BiologyMonash UniversityMelbourneAustralia

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