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Urothelial Cell Culture

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Wound Regeneration and Repair

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1037))

Abstract

This chapter reviews the use of urothelial cells as a means to enhance tissue regeneration and wound healing in urinary tract system. It addresses the properties of urothelial cells, including their role as a permeability barrier to protect underlying muscle tissue from the caustic effects of urine and as one of the main cell types, along with smooth muscle cells, that are used in urethral or bladder tissue engineering today. This description includes a general overview of various isolation techniques and culture methods that have been developed to improve urinary tract reconstruction in vivo and aid the characterization of growth factor expression in vitro. The chapter then describes various applications using urothelial cells, including production of multilayer urothelial sheets, tissue engineered bladder mucosa, tissue engineered urethra, and tissue engineered bladder. It also outlines the advantages of sandwich and layered coculture of these cells and the effects of epithelial–stromal cell interactions during tissue regeneration or wound healing processes in the urinary tract.

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Acknowledgments

The authors would like to thank Dr. Jennifer Olson for editorial assistance with this manuscript.

Author information

Authors and Affiliations

  1. Department of Urology, Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Winston-Salem, NC, USA

    Yuanyuan Zhang

  2. Department of Urology, Wake Forest Institute for Regenerative Medicine, Winston-Salem, NC, USA

    Anthony Atala

Authors
  1. Yuanyuan Zhang
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  2. Anthony Atala
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Editor information

Editors and Affiliations

  1. Virginia Tech Carilion Research Institut, Roanoke, Virginia, USA

    Robert G. Gourdie

  2. School of Pharmacy, MCPHS University, Worcester, Massachusetts, USA

    Tereance A. Myers

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Zhang, Y., Atala, A. (2013). Urothelial Cell Culture. In: Gourdie, R., Myers, T. (eds) Wound Regeneration and Repair. Methods in Molecular Biology, vol 1037. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-505-7_2

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  • DOI: https://doi.org/10.1007/978-1-62703-505-7_2

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-504-0

  • Online ISBN: 978-1-62703-505-7

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