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Evaluating B-Cells: From Bone Marrow Precursors to Antibody-Producing Cells

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Mouse Models of Allergic Disease

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1032))

Abstract

Lymphocyte characterization is primarily based on the differential expression of surface markers. In this context, flow-cytometry analysis (FACS) is an exceptional technique that not only allows the identification of B-cell subsets, but can also be used to evaluate cell function, activation, and division. Here, we will combine the use of FACS analysis and ELISA techniques to identify murine bone marrow and peripheral B-cell subsets. The main function of B cells, derived through a multistage differentiation process from precursor cells, is to produce antibodies. This task is performed by terminally differentiated B cells called antibody-secreting cells (ASC) present at mucosal sites, in the bone marrow and in the spleen. The number and specificity of ASC can be measured by Enzyme-linked immunosorbent spot (ELISPOT) assay, a variation of the enzyme-linked immunosorbent assay (ELISA) used to quantify serum immunoglobulins.

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Acknowledgments

We thank Dr. Claudio Pioli for his critical review of the manuscript. R.C. is supported by EUROPAD NET (EC Grant Nr. 201549).

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Rosado, M.M., Scarsella, M., Cascioli, S., Giorda, E., Carsetti, R. (2013). Evaluating B-Cells: From Bone Marrow Precursors to Antibody-Producing Cells. In: Allen, I. (eds) Mouse Models of Allergic Disease. Methods in Molecular Biology, vol 1032. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-496-8_4

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  • DOI: https://doi.org/10.1007/978-1-62703-496-8_4

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-495-1

  • Online ISBN: 978-1-62703-496-8

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