Abstract
T lymphocytes play positive and negative roles in the pathogenesis of allergic disease. Isolation and functional characterization of T lymphocyte subpopulations is an important aspect of understanding allergy models and allergy therapies. Measurement of the T cell surface proteins and T cell proliferation can provide insight into T cell activation. T cell function and the identities of T cell subsets can be determined by measuring cytokine production, either via intracellular cytokine staining or ELISPOT. This chapter outlines protocols for T cell isolation as well as the evaluation of surface protein expression, proliferation, intracellular cytokine staining, and ELISPOT.
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Barker, B.R. (2013). Evaluation of T Cell Function in Allergic Disease. In: Allen, I. (eds) Mouse Models of Allergic Disease. Methods in Molecular Biology, vol 1032. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-496-8_3
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DOI: https://doi.org/10.1007/978-1-62703-496-8_3
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-495-1
Online ISBN: 978-1-62703-496-8
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