Abstract
Thin-layer chromatography (TLC) is a technique that has been routinely used for the separation and identification of lipids. Here we describe an optimized protocol for the steady state labeling, separation, and quantification of yeast phospholipids using 1-D TLC.
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Acknowledgment
The Greenberg lab is supported by NIH grant DK081367, and R.M.D and A.S.J. are the recipients of Wayne State University Biology Department Graduate Enhancement grants.
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Deranieh, R.M., Joshi, A.S., Greenberg, M.L. (2013). Thin-Layer Chromatography of Phospholipids. In: Rapaport, D., Herrmann, J. (eds) Membrane Biogenesis. Methods in Molecular Biology, vol 1033. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-487-6_2
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DOI: https://doi.org/10.1007/978-1-62703-487-6_2
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-486-9
Online ISBN: 978-1-62703-487-6
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