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Membrane Biogenesis pp 219–251Cite as

Sedimentation Velocity Analytical Ultracentrifugation in Hydrogenated and Deuterated Solvents for the Characterization of Membrane Proteins

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1033))

Abstract

This chapter is a step-by-step protocol for setting up, realizing, and analyzing sedimentation velocity experiments in hydrogenated and deuterated solvents, in the context of the characterization of membrane protein, in terms of homogeneity, association state, and amount of bound detergent, based on a real case study of the membrane protein BmrA solubilized in n-Dodecyl-β-d-Maltopyranoside) detergent.

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Acknowledgments

This work used the AUC platform of the Grenoble Instruct centre (ISBG; UMS 3518 CNRS-CEA-UJF-EMBL) with support from FRISBI (ANR-10-INSB-05-02) and GRAL (ANR-10-LABX-49-01) within the Grenoble Partnership for Structural Biology (PSB).

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Authors and Affiliations

  1. Institut de Biologie Structurale, CEA, Grenoble, France

    Aline Le Roy, Hugues Nury, Benjamin Wiseman, Jonathan Sarwan, Jean-Michel Jault & Christine Ebel

Authors
  1. Aline Le Roy
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  2. Hugues Nury
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  3. Benjamin Wiseman
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  4. Jonathan Sarwan
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  5. Jean-Michel Jault
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  6. Christine Ebel
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Editor information

Editors and Affiliations

  1. Interfaculty Institute of Biochemistry, University of Tuebingen, Tuebingen, Germany

    Doron Rapaport

  2. Cell Biology, University of Kaiserslautern, Kaiserslautern, Germany

    Johannes M. Herrmann

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Le Roy, A., Nury, H., Wiseman, B., Sarwan, J., Jault, JM., Ebel, C. (2013). Sedimentation Velocity Analytical Ultracentrifugation in Hydrogenated and Deuterated Solvents for the Characterization of Membrane Proteins. In: Rapaport, D., Herrmann, J. (eds) Membrane Biogenesis. Methods in Molecular Biology, vol 1033. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-487-6_15

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  • DOI: https://doi.org/10.1007/978-1-62703-487-6_15

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  • Publisher Name: Humana Press, Totowa, NJ

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