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Lipid Peroxidation Due to In Vitro and In Vivo Exposure of Biological Samples to Nanoparticles

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Oxidative Stress and Nanotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1028))

Abstract

The increasing use of nanomaterials in biological applications raises numerous concerns about the dangers they might pose to living organisms. The rise in oxidative stress is usually the most readily observed effect induced by nanoparticles, with the measurement of lipid peroxidation levels being one of the most frequently used biological markers for its evaluation. Here, we describe the spectrophotometric and fluorimetric methods for determining the modifications of the malondialdehyde (MDA) level induced by many types of nanoparticles in in vitro and in vivo biological systems.

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Acknowledgement

This study was financially supported by the National Research Council of Higher Education, Romania, grant number 127TE/2010.

Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, University of Bucharest, Bucharest, Romania

    Anca Dinischiotu, Loredana Stanca, Sorina Nicoleta Petrache & Mihaela Radu

  2. Department of Biochemistry, Faculty of Pharmacology, Barol Davila, University of Medicine and Pharmacy, Bucharest, Romania

    Daniela Gradinaru

  3. Department of Preclinical Sciences, Agronouical Sciences and Vetrinary Medicine, Bucharest, Romania

    Andreea Iren Serban

Authors
  1. Anca Dinischiotu
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  2. Loredana Stanca
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  3. Daniela Gradinaru
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  4. Sorina Nicoleta Petrache
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  5. Mihaela Radu
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  6. Andreea Iren Serban
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Editor information

Editors and Affiliations

  1. University of Florida, n/a, Gainsville, 32611, Florida, USA

    Donald Armstrong

  2. , Pharmaceutical Research Institute, Albany College of Pharmacy, Discovery Lane 1, Rensselaer, 12144, USA

    Dhruba J Bharali

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Dinischiotu, A., Stanca, L., Gradinaru, D., Petrache, S.N., Radu, M., Serban, A.I. (2013). Lipid Peroxidation Due to In Vitro and In Vivo Exposure of Biological Samples to Nanoparticles. In: Armstrong, D., Bharali, D. (eds) Oxidative Stress and Nanotechnology. Methods in Molecular Biology, vol 1028. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-475-3_10

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  • DOI: https://doi.org/10.1007/978-1-62703-475-3_10

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-474-6

  • Online ISBN: 978-1-62703-475-3

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