Abstract
WaaL is a membrane enzyme that catalyzes the glycosidic bonding of a sugar at the proximal end of the undecaprenyl-diphosphate (Und-PP)-O-antigen with a terminal sugar of the lipid A-core oligosaccharide (OS). This is a critical step in lipopolysaccharide synthesis. We describe here an assay to perform the ligation reaction in vitro utilizing native substrates.
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Acknowledgments
This work was supported by a grant from the Canadian Institutes of Health Research to M.A.V., who also holds a Canada Research Chair in Infectious Diseases and Microbial Pathogenesis.
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Ruan, X., Valvano, M.A. (2013). In Vitro O-Antigen Ligase Assay. In: Brockhausen, I. (eds) Glycosyltransferases. Methods in Molecular Biology, vol 1022. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-465-4_15
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DOI: https://doi.org/10.1007/978-1-62703-465-4_15
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