Stability and Aggregation Assays of Nanoparticles in Biological Media

  • James Chen Yong Kah
Part of the Methods in Molecular Biology book series (MIMB, volume 1025)


Colloidal stability of nanoparticles in biological media is crucial to preserve their utility as aggregation often leads to undesirable biological response. A quantitative measurement of nanoparticles aggregation in solution would provide a valuable assessment of colloidal stability of bio–nano interfaces after surface functionalization. Here, we develop a quantitative technique based on optical absorption to assay the colloidal stability of plasmonic nanoparticles functionalized with different amphiphilic surface ligands in biologically relevant media.

Key words

Gold nanorods Colloidal stability Aggregation Amphiphilic ligand Surface charge Cell culture media 



This work was supported by the NSF grant DMR #0906838.


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • James Chen Yong Kah
    • 1
  1. 1.National University SingaporeSingaporeSingapore

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