Functionalization Protocols of Silicon Micro/Nano-mechanical Biosensors
Functionalization is a key element in biodetection technologies such as micro/nano-mechanical sensors. Since assay sensitivity and stability drastically depends on a proper bioreceptor immobilization, the sensing surface must be first chemically modified with uniform, well-packed, and robust layers. Here, we describe three functionalization protocols that we developed for the surface modification with amino, aldehyde, and carboxyl groups of micro/nano-mechanical biosensors.
Key wordsSelf-assembled monolayer Organosilane Functionalization Molecular recognition Biosensor
The authors wish to thank the financial support of Regione Piemonte (Converging Technologies 2007—NAMATECH grant, Poli di Innovazione POR-FESR 2007–2013—CANESTRO grant, and Piattaforme Innovative POR-FESR 2007–2013—ITACA and SAFE-FOOD CONTROL grants) and Italian Ministry of Research and Education (FIRB 2010—NEWTON grant, Progetti Bandiera 2011—NANOMAX grant).
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