Functionalization Protocols of Silicon Micro/Nano-mechanical Biosensors

  • Francesca Frascella
  • Carlo Ricciardi
Part of the Methods in Molecular Biology book series (MIMB, volume 1025)


Functionalization is a key element in biodetection technologies such as micro/nano-mechanical sensors. Since assay sensitivity and stability drastically depends on a proper bioreceptor immobilization, the sensing surface must be first chemically modified with uniform, well-packed, and robust layers. Here, we describe three functionalization protocols that we developed for the surface modification with amino, aldehyde, and carboxyl groups of micro/nano-mechanical biosensors.

Key words

Self-assembled monolayer Organosilane Functionalization Molecular recognition Biosensor 



The authors wish to thank the financial support of Regione Piemonte (Converging Technologies 2007—NAMATECH grant, Poli di Innovazione POR-FESR 2007–2013—CANESTRO grant, and Piattaforme Innovative POR-FESR 2007–2013—ITACA and SAFE-FOOD CONTROL grants) and Italian Ministry of Research and Education (FIRB 2010—NEWTON grant, Progetti Bandiera 2011—NANOMAX grant).


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Francesca Frascella
    • 1
  • Carlo Ricciardi
    • 1
  1. 1.Politecnico di TorinoTorinoItaly

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