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DNA Conjugation to Nanoparticles

  • Sunho Park
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1025)

Abstract

Nanoparticle–DNA (NP–DNA) conjugates have been highlighted due to their versatility in diverse science and engineering fields. The protocol of DNA conjugation to gold nanoparticles (AuNPs), which are among the most popular NPs in bio-applications, is thus described here. This protocol also includes ligand exchange of AuNP to make AuNPs suitable for conjugation process and a fluorescence technique to evaluate the average number of DNA strands attached to single AuNP.

Key words

Nanoparticle Gold nanoparticle DNA Conjugation Ligand exchange Gel electrophoresis Lyophilization DNA staining Fluorescence DNA coverage ratio 

References

  1. 1.
    Alivisatos P (2004) The use of nanocrystals in biological detection. Nat Biotechnol 22:47–52CrossRefGoogle Scholar
  2. 2.
    De M, Ghosh PS, Rotello VM (2008) Applications of nanoparticles in biology. Adv Mater 20:4225–4241CrossRefGoogle Scholar
  3. 3.
    Medintz IL, Uyeda HT, Goldman ER, Mattoussi H (2005) Quantum dot bioconjugates for imaging, labelling and sensing. Nat Mater 4:435–446CrossRefGoogle Scholar
  4. 4.
    Nel AE, Madler L, Velegol D, Xia T, Hoek EMV, Somasundaran P, Klaessig F, Castranova V, Thompson M (2009) Understanding biophysicochemical interactions at the nano-bio interface. Nat Mater 8:543–557CrossRefGoogle Scholar
  5. 5.
    Choi HS, Liu W, Liu F, Nasr K, Misra P, Bawendi MG, Frangioni JV (2010) Design considerations for tumor-targeted nanoparticles. Nat Nanotechnol 5:42–47CrossRefGoogle Scholar
  6. 6.
    Chen C-C, Lin Y-P, Wang C-W, Tzeng H-C, Wu C-H, Chen Y-C, Chen C-P, Chen L-C, Wu Y-C (2006) DNA-gold nanorod conjugates for remote control of localized gene expression by near infrared irradiation. J Am Chem Soc 128:3709–3715CrossRefGoogle Scholar
  7. 7.
    Ghosh PS, Kim C-K, Han G, Forbes NS, Rotello VM (2008) Efficient gene delivery vectors by tuning the surface charge density of amino acid-functionalized gold nanoparticles. ACS Nano 2:2213–2218CrossRefGoogle Scholar
  8. 8.
    Thomas M, Klibanov AM (2003) Conjugation to gold nanoparticles enhances polyethylenimine’s transfer of plasmid DNA into mammalian cells. Proc Natl Acad Sci U S A 100:9138–9143CrossRefGoogle Scholar
  9. 9.
    Macfarlane RJ, Lee B, Jones MR, Harris N, Schatz GC, Mirkin CA (2011) Nanoparticle superlattice engineering with DNA. Science 334:204–208CrossRefGoogle Scholar
  10. 10.
    Frens G (1973) Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions. Nat Phys Sci 241:20–22CrossRefGoogle Scholar
  11. 11.
    Park S, Brown KA, Hamad-Schifferli K (2004) Changes in oligonucleotide conformation on nanoparticle surfaces by modification with mercaptohexanol. Nano Lett 4:1925–1929CrossRefGoogle Scholar
  12. 12.
    Dulkeith E, Ringler M, Klar TA, Feldmann J (2005) Gold nanoparticles quench fluorescence by phase induced radiative rate suppression. Nano Lett 5:585–589CrossRefGoogle Scholar
  13. 13.
    Liu X, Atwater M, Wang J, Qun H (2007) Extinction coefficient of gold nanoparticles with different sizes and different capping ligands. Colloids Surf B 58:3–7CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Sunho Park
    • 1
  1. 1.Dankook UniversityYongin-siSouth Korea

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