DNA Conjugation to Nanoparticles

  • Sunho Park
Part of the Methods in Molecular Biology book series (MIMB, volume 1025)


Nanoparticle–DNA (NP–DNA) conjugates have been highlighted due to their versatility in diverse science and engineering fields. The protocol of DNA conjugation to gold nanoparticles (AuNPs), which are among the most popular NPs in bio-applications, is thus described here. This protocol also includes ligand exchange of AuNP to make AuNPs suitable for conjugation process and a fluorescence technique to evaluate the average number of DNA strands attached to single AuNP.

Key words

Nanoparticle Gold nanoparticle DNA Conjugation Ligand exchange Gel electrophoresis Lyophilization DNA staining Fluorescence DNA coverage ratio 


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Sunho Park
    • 1
  1. 1.Dankook UniversityYongin-siSouth Korea

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