Electrophoretic Implementation of the Solution-Depletion Method for Measuring Protein Adsorption, Adsorption Kinetics, and Adsorption Competition Among Multiple Proteins in Solution
The venerable solution-depletion method is perhaps the most unambiguous method of measuring solute adsorption from solution to solid particles, requiring neither complex instrumentation nor associated interpretive theory. We describe herein an SDS-gel electrophoresis implementation of the solution-depletion method for measuring protein adsorption and protein-adsorption kinetics. Silanized-glass particles with different surface chemistry/energy and hydrophobic sepharose-based chromatographic media are used as example adsorbents. Electrophoretic separation enables quantification of adsorption competition among multiple proteins in solution for the same adsorbent surface, demonstrated herein by adsorption-competition kinetics from binary solution.
Key wordsProtein adsorption Adsorption kinetics Adsorption competition Electrophoresis Solution depletion
This work was supported by National Institute of Health grant PHS 5R01HL069965. The authors appreciate the support from the Departments of Materials Science and Engineering and Bioengineering, The Pennsylvania State University.
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