Preparation of 2 nm Gold Nanoparticles for In Vitro and In Vivo Applications

  • Daniel F. Moyano
  • Bradley Duncan
  • Vincent M. Rotello
Part of the Methods in Molecular Biology book series (MIMB, volume 1025)


Gold nanoparticles have been a versatile tool in recent years for the exploration of biological systems. However, challenges with purification and adequate surface coverage limit the biocompatibility of gold nanoparticles. Here, we describe a detailed procedure for the synthesis, purification, and functionalization of biologically compatible gold nanoparticles for in vitro and in vivo studies.

Key words

Gold nanoparticle Biocompatibility Purification 



This work was supported by the NIH (GM077173 and EB014277).


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Daniel F. Moyano
    • 1
  • Bradley Duncan
    • 1
  • Vincent M. Rotello
    • 1
  1. 1.University of MassachusettsAmherstUSA

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