Abstract
MicroRNAs (miRNAs) are small regulatory RNA molecules that modulate specific target mRNAs and play very important roles in physiological processes. They were recently detected in body fluids such as blood, urine, saliva, and milk. These body fluid miRNAs have been studied thoroughly as potential diagnostic biomarkers. However, there have been few studies of milk miRNAs, and their roles are not clearly understood. Milk is the only nutritional source for newborn infants, and bovine milk is used widely as a dairy product. Thus, it is important to study milk miRNAs. In general, body fluid RNA concentrations are extremely low and of diverse existence types. In this chapter, we compare two silica membrane column-based RNA purification kits, and also compare RNA obtained directly from whey with that isolated from whey-derived exosomes.
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References
Welch RAS, Burns DJW, Davis SR et al (1997) Milk composition, production and biotechnology. CAB International, Oxon
Jensen RG (1995) Handbook of milk composition. Academic, San Diego
Nollet LML, Toldrá F (2009) Handbook of dairy foods analysis. CRC, Boca Raton
Walker A (2010) Breast milk as the gold standard for protective nutrients. J Pediatr 156:S3–S7
Goldman AS (2007) The immune system in human milk and the developing infant. Breastfeed Med 2:195–204
Kosaka N, Izumi H, Sekine K et al (2010) microRNA as a new immune-regulatory agent in breast milk. Silence 1:7
Weber JA, Baxter DH, Zhang S et al (2010) The microRNA spectrum in 12 body fluids. Clin Chem 56:1733–1741
Hata T, Murakami K, Nakatani H et al (2010) Isolation of bovine milk-derived microvesicles carrying mRNAs and microRNAs. Biochem Biophys Res Commun 396:528–533
Chen X, Gao C, Li H et al (2010) Identification and characterization of microRNAs in raw milk during different periods of lactation, commercial fluid, and powdered milk products. Cell Res 20:1128–1137
Lässer C, Alikhani VS, Ekström K et al (2011) Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages. J Transl Med 9:9
Zhou Q, Li M, Wang X et al (2012) Immune-related microRNAs are abundant in breast milk exosomes. Int J Biol Sci 8:118–123
Izumi H, Kosaka N, Shimizu T et al (2012) Bovine milk contains microRNA and messenger RNA that are stable under degradative conditions. J Dairy Sci 95:4831–4841
Lewis BP, Shih IH, Jones-Rhoades MW et al (2003) Prediction of mammalian microRNA targets. Cell 115:787–798
Mitchell PAR, Parkin RK, Kroh EM et al (2008) Circulating microRNAs as stable blood-based markers for cancer detection. Proc Natl Acad Sci U S A 105:10513–10518
Hanke M, Hoefig K, Merz H et al (2010) A robust methodology to study urine microRNA as a tumor marker: microRNA-126 and microRNA-182 are related to urinary bladder cancer. Urol Oncol 28:655–661
Michael A, Bajracharya SD, Yuen PS et al (2010) Exosomes from human saliva as a source of microRNA biomarkers. Oral Dis 16:34–38
Arroyo JD, Chevillet JR, Kroh EM et al (2011) Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma. Proc Natl Acad Sci U S A 108:5003–5008
Valadi H, Ekström K, Bossios A et al (2007) Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat Cell Biol 9:654–659
Simpson RJ, Lim JW, Moritz RL et al (2009) Exosomes: proteomic insights and diagnostic potential. Expert Rev Proteomics 6:267–283
Admyre C, Johansson SM, Qazi KR et al (2007) Exosomes with immune modulatory features are present in human breast milk. J Immunol 179:1969–1978
Zhang L, Hou D, Chen X et al (2012) Exogenous plant MIR168a specifically targets mammalian LDLRAP1: evidence of cross-kingdom regulation by microRNA. Cell Res 22:107–126
Maningat PD, Sen P, Rijnkels M et al (2009) Gene expression in the human mammary Âepithelium during lactation: the milk fat globule transcriptome. Physiol Genomics 37:12–22
Luckey TD, Mende TJ, Pleasants J (1954) The physical and chemical characterization of rat’s milk. J Nutr 54:345–359
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Izumi, H., Kosaka, N., Shimizu, T., Sekine, K., Ochiya, T., Takase, M. (2013). Purification of RNA from Milk Whey. In: Kosaka, N. (eds) Circulating MicroRNAs. Methods in Molecular Biology, vol 1024. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-453-1_15
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DOI: https://doi.org/10.1007/978-1-62703-453-1_15
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-62703-453-1
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