Abstract
In higher plants guanylyl cyclases (GCs) and adenylyl cyclases (ACs) cannot be identified using BLAST homology searches based on annotated cyclic nucleotide cyclases (CNCs) of prokaryotes, lower eukaryotes, or animals. The reason is that CNCs are often part of complex multifunctional proteins with different domain organizations and biological functions that are not conserved in higher plants. For this reason, we have developed CNC search strategies based on functionally conserved amino acids in the catalytic center of annotated and/or experimentally confirmed CNCs. Here we detail this method which has led to the identification of >25 novel candidate CNCs in Arabidopsis thaliana, several of which have been experimentally confirmed in vitro and in vivo. We foresee that the application of this method can be used to identify many more members of the growing family of CNCs in higher plants.
Key words
- Cyclic nucleotide cyclase
- Adenylyl cyclase
- cAMP
- Guanylyl cyclase
- cGMP
- Catalytic center
- Motif search
- Homology modeling
- Basic local alignment search tool
- Arabidopsis thaliana
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Wong, A., Gehring, C. (2013). Computational Identification of Candidate Nucleotide Cyclases in Higher Plants. In: Gehring, C. (eds) Cyclic Nucleotide Signaling in Plants. Methods in Molecular Biology, vol 1016. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-441-8_13
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DOI: https://doi.org/10.1007/978-1-62703-441-8_13
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